Mercurial > repos > galaxytrakr > hfp_bettercallsal_awsbatch
diff 1.0.0/modules/minimap2/align/main.nf @ 0:801b85b03a17 draft default tip
planemo upload
| author | galaxytrakr |
|---|---|
| date | Thu, 28 May 2026 20:31:42 +0000 |
| parents | |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/1.0.0/modules/minimap2/align/main.nf Thu May 28 20:31:42 2026 +0000 @@ -0,0 +1,52 @@ +process MINIMAP2_ALIGN { + tag "$meta.id" + label 'process_micro' + + module (params.enable_module ? + "${params.swmodulepath}${params.fs}minimap2${params.fs}2.22:${params.swmodulepath}${params.fs}samtools${params.fs}1.13" : null) + conda (params.enable_conda ? "bioconda::minimap2=2.24 bioconda::samtools=1.18 conda-forge::perl" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:365b17b986c1a60c1b82c6066a9345f38317b763-0' : + 'quay.io/biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:365b17b986c1a60c1b82c6066a9345f38317b763-0' }" + + input: + tuple val(meta), path(reads) + tuple val(meta2), path(reference) + val bam_format + val align_bam_sorted + val cigar_paf_format + val cigar_bam + + output: + tuple val(meta), path("*.paf"), emit: paf, optional: true + tuple val(meta), path("*.bam"), emit: bam, optional: true + tuple val(meta), path("*.bai"), emit: bai, optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def bam_sort = align_bam_sorted ? "samtools sort | samtools view -@${task.cpus} -b -h -o ${prefix}.bam; samtools index -@${task.cpus} ${prefix}.bam" : "samtools view -@${task.cpus} -b -h -o ${prefix}.bam" + def bam_output = bam_format ? "-a | ${bam_sort}" : "-o ${prefix}.paf" + def cigar_paf = cigar_paf_format && !bam_format ? "-c" : '' + def set_cigar_bam = cigar_bam && bam_format ? "-L" : '' + """ + minimap2 \\ + $args \\ + -t $task.cpus \\ + "${reference ?: reads}" \\ + "$reads" \\ + $cigar_paf \\ + $set_cigar_bam \\ + $bam_output + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + minimap2: \$(minimap2 --version 2>&1) + samtools: \$(samtools version | head -n1 | sed -e 's/samtools //' 2>&1) + END_VERSIONS + """ +} \ No newline at end of file
