Mercurial > repos > galaxytrakr > hfp_bettercallsal_konda
comparison 1.0.0/modules/minimap2/align/main.nf @ 0:0a8dda29956e draft default tip
planemo upload
| author | galaxytrakr |
|---|---|
| date | Thu, 28 May 2026 20:41:10 +0000 |
| parents | |
| children |
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| -1:000000000000 | 0:0a8dda29956e |
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| 1 process MINIMAP2_ALIGN { | |
| 2 tag "$meta.id" | |
| 3 label 'process_micro' | |
| 4 | |
| 5 module (params.enable_module ? | |
| 6 "${params.swmodulepath}${params.fs}minimap2${params.fs}2.22:${params.swmodulepath}${params.fs}samtools${params.fs}1.13" : null) | |
| 7 conda (params.enable_conda ? "bioconda::minimap2=2.24 bioconda::samtools=1.18 conda-forge::perl" : null) | |
| 8 container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? | |
| 9 'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:365b17b986c1a60c1b82c6066a9345f38317b763-0' : | |
| 10 'quay.io/biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:365b17b986c1a60c1b82c6066a9345f38317b763-0' }" | |
| 11 | |
| 12 input: | |
| 13 tuple val(meta), path(reads) | |
| 14 tuple val(meta2), path(reference) | |
| 15 val bam_format | |
| 16 val align_bam_sorted | |
| 17 val cigar_paf_format | |
| 18 val cigar_bam | |
| 19 | |
| 20 output: | |
| 21 tuple val(meta), path("*.paf"), emit: paf, optional: true | |
| 22 tuple val(meta), path("*.bam"), emit: bam, optional: true | |
| 23 tuple val(meta), path("*.bai"), emit: bai, optional: true | |
| 24 path "versions.yml" , emit: versions | |
| 25 | |
| 26 when: | |
| 27 task.ext.when == null || task.ext.when | |
| 28 | |
| 29 script: | |
| 30 def args = task.ext.args ?: '' | |
| 31 def prefix = task.ext.prefix ?: "${meta.id}" | |
| 32 def bam_sort = align_bam_sorted ? "samtools sort | samtools view -@${task.cpus} -b -h -o ${prefix}.bam; samtools index -@${task.cpus} ${prefix}.bam" : "samtools view -@${task.cpus} -b -h -o ${prefix}.bam" | |
| 33 def bam_output = bam_format ? "-a | ${bam_sort}" : "-o ${prefix}.paf" | |
| 34 def cigar_paf = cigar_paf_format && !bam_format ? "-c" : '' | |
| 35 def set_cigar_bam = cigar_bam && bam_format ? "-L" : '' | |
| 36 """ | |
| 37 minimap2 \\ | |
| 38 $args \\ | |
| 39 -t $task.cpus \\ | |
| 40 "${reference ?: reads}" \\ | |
| 41 "$reads" \\ | |
| 42 $cigar_paf \\ | |
| 43 $set_cigar_bam \\ | |
| 44 $bam_output | |
| 45 | |
| 46 cat <<-END_VERSIONS > versions.yml | |
| 47 "${task.process}": | |
| 48 minimap2: \$(minimap2 --version 2>&1) | |
| 49 samtools: \$(samtools version | head -n1 | sed -e 's/samtools //' 2>&1) | |
| 50 END_VERSIONS | |
| 51 """ | |
| 52 } |
