Mercurial > repos > galaxytrakr > hfp_cronology_awsbatch
comparison 0.2.0/modules/spades/assemble/README.md @ 0:9e8b1c747a6a draft default tip
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| author | galaxytrakr |
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| date | Fri, 29 May 2026 13:32:17 +0000 |
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| -1:000000000000 | 0:9e8b1c747a6a |
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| 1 # NextFlow DSL2 Module | |
| 2 | |
| 3 ```bash | |
| 4 SPADES_ASSEMBLE | |
| 5 ``` | |
| 6 | |
| 7 ## Description | |
| 8 | |
| 9 Run `spades` assembler tool on a list of read files in FASTQ format. | |
| 10 | |
| 11 \ | |
| 12 | |
| 13 | |
| 14 ### `input:` | |
| 15 | |
| 16 ___ | |
| 17 | |
| 18 Type: `tuple` | |
| 19 | |
| 20 Takes in the following tuple of metadata (`meta`) and a list of FASTQ files from various platforms of input type `path` (`illumina`, `pacbio`, `nanopore`). | |
| 21 | |
| 22 Ex: | |
| 23 | |
| 24 ```groovy | |
| 25 [ [id: 'sample1', single_end: true], '/data/sample1/f_merged.fq.gz' ] | |
| 26 [ [id: 'sample1', single_end: false], ['/data/sample1/f1_merged.fq.gz', '/data/sample2/f2_merged.fq.gz'], ['/data/sample1/nanopore.fastq'], ['/data/sample1/pacbio.fastq'] ] | |
| 27 ``` | |
| 28 | |
| 29 \ | |
| 30 | |
| 31 | |
| 32 #### `meta` | |
| 33 | |
| 34 Type: Groovy Map | |
| 35 | |
| 36 A Groovy Map containing the metadata about the FASTQ file. | |
| 37 | |
| 38 Ex: | |
| 39 | |
| 40 ```groovy | |
| 41 [ id: 'FAL00870', strandedness: 'unstranded', single_end: true ] | |
| 42 ``` | |
| 43 | |
| 44 \ | |
| 45 | |
| 46 | |
| 47 #### `illumina` | |
| 48 | |
| 49 Type: `path` | |
| 50 | |
| 51 NextFlow input type of `path` pointing to Illumina read files in FASTQ format that need to be *de novo* assembled along with reads from any other sequencing platforms, if any. | |
| 52 | |
| 53 \ | |
| 54 | |
| 55 | |
| 56 #### `nanopore` | |
| 57 | |
| 58 Type: `path` | |
| 59 | |
| 60 NextFlow input type of `path` pointing to Oxford Nanopore read files in FASTQ format that need to be *de novo* assembled along with reads from any other sequencing platforms, if any. | |
| 61 | |
| 62 \ | |
| 63 | |
| 64 | |
| 65 #### `pacbio` | |
| 66 | |
| 67 Type: `path` | |
| 68 | |
| 69 NextFlow input type of `path` pointing to PacBio read files in FASTQ format that need to be *de novo* assembled along with reads from any other sequencing platforms, if any. | |
| 70 | |
| 71 \ | |
| 72 | |
| 73 | |
| 74 #### `args` | |
| 75 | |
| 76 Type: Groovy String | |
| 77 | |
| 78 String of optional command-line arguments to be passed to the tool. This can be mentioned in `process` scope within `withName:process_name` block using `ext.args` option within your `nextflow.config` file. | |
| 79 | |
| 80 Ex: | |
| 81 | |
| 82 ```groovy | |
| 83 withName: 'SPADES_ASSEMBLE' { | |
| 84 ext.args = '--rna' | |
| 85 } | |
| 86 ``` | |
| 87 | |
| 88 \ | |
| 89 | |
| 90 | |
| 91 ### `output:` | |
| 92 | |
| 93 ___ | |
| 94 | |
| 95 Type: `tuple` | |
| 96 | |
| 97 Outputs a tuple of metadata (`meta` from `input:`) and `spades` assembled scaffolds file in FASTA format. | |
| 98 | |
| 99 \ | |
| 100 | |
| 101 | |
| 102 #### `assembly` | |
| 103 | |
| 104 Type: `path` | |
| 105 | |
| 106 NextFlow output type of `path` pointing to the `spades` assembler results file (`scaffolds.fasta`) per sample (`id:`) i.e., the final assembled scaffolds file in FASTA format. | |
| 107 | |
| 108 \ | |
| 109 | |
| 110 | |
| 111 #### `versions` | |
| 112 | |
| 113 Type: `path` | |
| 114 | |
| 115 NextFlow output type of `path` pointing to the `.yml` file storing software versions for this process. |
