jasmine_amir@0: The Galaxy tool wrappers contained in this tool shed repository rely on software developed by jasmine_amir@0: the NCBI: https://github.com/ncbi/sra-tools. jasmine_amir@0: jasmine_amir@0: NCBI Sequence Read Archive Toolkit: https://trace.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?view=software jasmine_amir@0: jasmine_amir@0: # Installation of Aspera connect ascp binary jasmine_amir@0: jasmine_amir@0: The sra-tools suite is ready to benefit from increased transfer speed and reliability by using Aspera Connect ascp. jasmine_amir@0: To benefit, download the ascp commandline client, and place ascp and the required ssh keys into a PATH accessible to galaxys job handler. jasmine_amir@0: jasmine_amir@0: A convenience package for linux and OS X is available at https://toolshed.g2.bx.psu.edu/view/mvdbeek/package_ascp_3/e109f0ec22c3 . jasmine_amir@0: It suffices to copy the contents of the $INSTALL_DIR/bin into PATH. jasmine_amir@0: jasmine_amir@0: Alternatively go to http://downloads.asperasoft.com/connect2/ . jasmine_amir@0: jasmine_amir@0: Aspera connect is not provided by the IUC due to its closed-source nature. jasmine_amir@0: jasmine_amir@0: # Firewall settings for highspeed transfer jasmine_amir@0: jasmine_amir@0: To benefit from increased transfer speeds using ascp3 your local firewall must permit UDP data transfer in both jasmine_amir@0: directions on ports 33001-33009 for the following IP ranges: jasmine_amir@0: jasmine_amir@0: 130.14.*.* jasmine_amir@0: jasmine_amir@0: 165.112.*.* jasmine_amir@0: jasmine_amir@0: The firewall must also allow ssh traffic outbound to NCBI. jasmine_amir@0: The wrapper will fall back to http download if these requirements are not met. jasmine_amir@0: jasmine_amir@0: CONTROLLED-ACCESS DATA jasmine_amir@0: jasmine_amir@0: Encrypted, controlled-access data is not supported.