Mercurial > repos > jpayne > bam_dealer
comparison paired_bam_dealer.xml @ 4:881a24a35480
planemo upload commit b'f16e028df365af5bc6b4b441152d278a55e40ffe\n'-dirty
author | jpayne |
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date | Wed, 06 Dec 2017 16:41:46 -0500 |
parents | b7bbe08d50df |
children | 55df0d1c6365 |
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3:b7bbe08d50df | 4:881a24a35480 |
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1 <tool id="paired_bam_dealer" name="Deal out" version="0.1.0"> | 1 <tool id="paired_bam_dealer" name="Deal out" version="0.1.0"> |
2 <description>paired-end reads by alignment or non-alignment to a reference</description> | 2 <description>paired-end reads by alignment or non-alignment to a reference</description> |
3 <requirements> | 3 <requirements> |
4 <requirement type="Tool_Shed_Package" version="1.5">samtools</requirement> | 4 <requirement type="package" version="1.5">samtools</requirement> |
5 <requirement type="Tool_Shed_Package" version="2.26">bedtools</requirement> | 5 <requirement type="package" version="2.26">bedtools</requirement> |
6 </requirements> | 6 </requirements> |
7 <command detect_errors="exit_code"><![CDATA[ | 7 <command detect_errors="exit_code"><![CDATA[ |
8 bamToFastq -i <(samtools sort -n <(samtools view -u -f 1 -F 12 $input_bam )) | 8 which -a samtools ; ldd `which samtools` |
9 -fq $mapped_1 | |
10 -fq2 $mapped_2 && echo "mapped out" && | |
11 bamToFastq -i <( | |
12 samtools merge -u - | |
13 <(samtools view -u -f 4 -F 264 $input_bam) | |
14 <(samtools view -u -f 8 -F 260 $input_bam) | |
15 <(samtools view -u -f 12 -F 256 $input_bam) | |
16 | samtools sort -n | |
17 ) | |
18 -fq $unmapped_1 | |
19 -fq2 $unmapped_2 && echo "unmapped out" | |
20 ]]></command> | 9 ]]></command> |
21 <inputs> | 10 <inputs> |
22 <param format="bam" name="input_bam" type="data" label="BAM alignment" /> | 11 <param format="bam" name="input_bam" type="data" label="BAM alignment" /> |
23 </inputs> | 12 </inputs> |
24 <outputs> | 13 <outputs> |