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"ConFindr initial commit"
author | jpayne |
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date | Thu, 24 Sep 2020 13:39:12 -0400 |
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<tool id="confindr" name="ConFindr" version="0.1.0"> <requirements> <requirement type="package" version="0.7.4">confindr</requirement> </requirements> <command detect_errors="exit_code"><![CDATA[ ## Copy and optionally uncompress reads ## #if $reads.reads_select == 'collection' #set forward=$reads.coll.forward #set reverse=$reads.coll.reverse #set name=$reads.coll.name.replace(' ', '_') #else #set name=$forward.name.split('_')[0] #end if mkdir ./${name} #if $forward.is_of_type("fastq.gz","fastqsanger.gz") && cp $forward ./${name}/${name}_R1.fastq.gz #else if $forward.is_of_type("fastq.bz2", "fastqsanger.bz2") && tar -vxjOf $forward > ./${name}/${name}_R1.fastq #else && cp $forward ./${name}/${name}_R1.fastq #end if #if $reverse.is_of_type("fastq.gz","fastqsanger.gz") && cp $reverse ./${name}/${name}_R2.fastq.gz #else if $reverse.is_of_type("fastq.bz2", "fastqsanger.bz2") && tar -vxjOf $reverse > ./${name}/${name}_R2.fastq #else && cp $reverse ./${name}/${name}_R2.fastq #end if ## !!! confindr in single-threaded mode; race condition bugs in multiple threads !!! ## && confindr.py -i ./${name} -o ./output -t 1 && cat ./output/confindr_log.txt && ls -lah ./output/confindr_report.csv ]]></command> <inputs> <conditional name="reads"> <param name="reads_select" type="select" label="Paired-end collection, or two datasets from your history"> <option value="collection">Paired collection from your history</option> <option value="history">Two FASTQ datasets from your history</option> </param> <when value="collection"> <param label="Paired reads" name="coll" type="data_collection" format="fastq,fastqsanger,fastq.gz,fastqsanger.gz,fastq.bz2,fastqsanger.bz2" collection_type="paired" /> </when> <when value="history"> <param label="Forward reads" type="data" name="forward" format="fastq,fastqsanger,fastq.gz,fastqsanger.gz,fastq.bz2,fastqsanger.bz2" /> <param label="Reverse reads" type="data" name="reverse" format="fastq,fastqsanger,fastq.gz,fastqsanger.gz,fastq.bz2,fastqsanger.bz2" /> </when> </conditional> </inputs> <outputs> <data format="csv" name="contam_report" label="Contamination Report" from_work_dir="./output/confindr_report.csv" /> </outputs> <tests> <test> <param name="reads_select" value="collection" /> <param name="coll"> <collection type="paired"> <element name="forward" value="example_R1.fastq.gz" ftype="fastqsanger.gz" /> <element name="reverse" value="example_R2.fastq.gz" ftype="fastqsanger.gz" /> </collection> </param> <output name="contam_report" value="confindr_report.csv" /> </test> <test> <param name="reads_select" value="history" /> <param name="forward" value="example_R1.fastq.gz" ftype="fastqsanger.gz"/> <param name="reverse" value="example_R2.fastq.gz" ftype="fastqsanger.gz"/> <output name="contam_report" value="confindr_report_2.csv" /> </test> <test> <param name="reads_select" value="history" /> <param name="forward" value="example_R1.fastq.bz2" ftype="fastqsanger.bz2"/> <param name="reverse" value="example_R2.fastq.bz2" ftype="fastqsanger.bz2"/> <output name="contam_report" value="confindr_report_2.csv" /> </test> </tests> <help><![CDATA[ ConFindr, a contamination screen for Illumina sequencing ]]></help> <citations> <citation type="doi">10.7717/peerj.6995</citation> <citation type="bibtex"> @misc{githubConFindr, author = {LastTODO, FirstTODO}, year = {TODO}, title = {ConFindr}, publisher = {GitHub}, journal = {GitHub repository}, url = {https://github.com/OLC-Bioinformatics/ConFindr}, }</citation> </citations> </tool>