Mercurial > repos > jpayne > seqsero_v2
diff SeqSero2/SalmID/README.md @ 12:4ac593d4b40f
planemo upload
author | jpayne |
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date | Wed, 14 Aug 2019 17:45:41 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/SeqSero2/SalmID/README.md Wed Aug 14 17:45:41 2019 -0400 @@ -0,0 +1,49 @@ +[![DOI](https://zenodo.org/badge/97020646.svg)](https://zenodo.org/badge/latestdoi/97020646) + +# SalmID +Rapid tool to check taxonomic ID of single isolate samples. Currently only IDs Salmonella species and subspecies, and some common contaminants (Listeria, Escherichia). + +## Requirements: +Python 3 + +## Installation: +The easy way with homebrew ([Linux](http://linuxbrew.sh/) or [MacOS](https://brew.sh/)): +``` +brew install brewsci/bio/salmid +``` +Big thanks to [Torsten Seemann](https://tseemann.github.io/) for including this in homebrew! + +Alernatively git clone to your machine: +``` +git clone --recursive https://github.com/hcdenbakker/SalmID.git +``` + +Make SalmID executable: +``` +cd SalmID +``` + +``` +chmod +x SalmID.py +``` + + +Add the SalmID folder to your path + +To execute: +``` +SalmID.py -e .fastq.gz +``` +This will perform a SalmID run on all fastq.gz files in the current directory. +``` +SalmID.py -i your_fastq_gz.fastq.gz +``` +This will perform a SalmID run on an individual file (i.e., your_fastq_gz.fastq.gz) +``` +SalmID.py -d directory_with_data -e _1.fastq.gz +``` +This will perform a SalmID run on all files in directory 'directory_with_data' with extension '_1.fastq.gz' + +## Todo's and thoughts for future releases: +- Provide coverage estimates for genomes in sample based on kmer frequencies +- Write code to use SalmID on long read (minion, pacbio) platforms