Mercurial > repos > jpayne > seqsero_v2
view SeqSero2/SalmID/README.md @ 16:3b6d5b60968f tip
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author | estrain |
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date | Wed, 01 Mar 2023 13:21:51 -0500 |
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[![DOI](https://zenodo.org/badge/97020646.svg)](https://zenodo.org/badge/latestdoi/97020646) # SalmID Rapid tool to check taxonomic ID of single isolate samples. Currently only IDs Salmonella species and subspecies, and some common contaminants (Listeria, Escherichia). ## Requirements: Python 3 ## Installation: The easy way with homebrew ([Linux](http://linuxbrew.sh/) or [MacOS](https://brew.sh/)): ``` brew install brewsci/bio/salmid ``` Big thanks to [Torsten Seemann](https://tseemann.github.io/) for including this in homebrew! Alernatively git clone to your machine: ``` git clone --recursive https://github.com/hcdenbakker/SalmID.git ``` Make SalmID executable: ``` cd SalmID ``` ``` chmod +x SalmID.py ``` Add the SalmID folder to your path To execute: ``` SalmID.py -e .fastq.gz ``` This will perform a SalmID run on all fastq.gz files in the current directory. ``` SalmID.py -i your_fastq_gz.fastq.gz ``` This will perform a SalmID run on an individual file (i.e., your_fastq_gz.fastq.gz) ``` SalmID.py -d directory_with_data -e _1.fastq.gz ``` This will perform a SalmID run on all files in directory 'directory_with_data' with extension '_1.fastq.gz' ## Todo's and thoughts for future releases: - Provide coverage estimates for genomes in sample based on kmer frequencies - Write code to use SalmID on long read (minion, pacbio) platforms