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author | jpayne |
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date | Wed, 31 Jan 2018 12:32:01 -0500 |
parents | eefdd97a6749 |
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<tool id="index_ref" name="0. Get or Build Index" version="1.0.1" profile="16.10"> <description>by lookup from BioProject or from an assembly</description> <requirements> <requirement type="package" version="2.3.4">bowtie2</requirement> <requirement type="package" version="1.6">samtools</requirement> <requirement type="package" version="1.5.11">boto3</requirement> </requirements> <command detect_errors="exit_code"><![CDATA[ #if $source.source_select == 'bioproject' ${__tool_directory__}/snp-cache.py snp_bioproject_references "${bioproject}" -o ${output} && ${__tool_directory__}/snp-cache.py snp_bioproject_indexes "${bioproject}" -o ${faidx} #else mkdir ./reference && cp ${input} ./reference/reference.fasta && export ID=\$(md5sum $input | cut -c -32) && ${__tool_directory__}/snp-cache.py snp_local_references \$ID -c " bowtie2-build ./reference/reference.fasta --quiet --threads \${GALAXY_SLOTS:-4} ./reference/reference && tar -zcf /dev/stdout ./reference/ref*bt2 " -o ${output} -l $cache_log ##&& ${__tool_directory__}/snp-cache.py snp_local_indexes \$ID ## -c " ## samtools faidx ./reference/reference.fasta && cat ./reference/reference.fasta.fai ## " ## -o ${faidx} #end if && cat $cache_log ]]></command> <inputs> <conditional name="source"> <param name="source_select" type="select" label="Use the reference associated with a provided BioProject, a curated GalaxyTrakr reference, or a reference from your history"> <option value="bioproject">Provide a BioProject</option> <option value="curated">Use a GalaxyTrakr reference</option> <option value="history">Use a reference from your history</option> </param> <when value="bioproject"> <param type="data" name="bioproject" format="text"/> </when> <when value="curated"> <param name="input" type="select" label="Select reference fasta"> <options from_data_table="all_fasta"> <filter type="sort_by" column="2"/> <validator type="no_options" message="No assemblies are available for the selected input dataset"/> </options> </param> </when> <when value="history"> <param type="data" name="input" format="fasta" label="Select reference FASTA"/> </when> </conditional> </inputs> <outputs> <data label="Indexed reference (bt2)" name="output" format="tar" /> <!-- <data label="Indexed reference (faidx)" name="faidx" format="fai" /> --> <data label="S3 Cache log" name="cache_log" format="txt" hidden="true" /> </outputs> <tests> <test> <param name="source_select" value="history" /> <param name="input" value="reference/lambda_virus.fasta" /> <output name="output" value="reference/reference.tar.gz" decompress="true" lines_diff="10" /> <!-- <output name="faidx" value="reference/reference.fasta.fai" /> --> </test> </tests> <help><![CDATA[ <a href="http://snp-pipeline.readthedocs.io/en/latest/index.html">http://snp-pipeline.readthedocs.io/en/latest/index.html</a> ]]></help> <citations> <citation type="doi">10.7717/peerj-cs.20</citation> <!-- <citation type="bibtex"> @misc{cfsan-snp-pipeline, author = {Steve Davis and James Pettengill and Yan Luo and Justin Payne and Albert Shpuntoff and Rugh Rand and Errol Strain}, year = {2015}, title = {CFSAN SNP Pipeline: an automated method for constructing SNP matrices from next-generation sequence data}, url = {https://doi.org/10.7717/peerj-cs.20}, journal = {PeerJ Computer Science}, }</citation> --> </citations> </tool>