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view 5_call_consensus.xml @ 25:f986ff235d69
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author | jpayne |
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date | Wed, 31 Jan 2018 13:13:17 -0500 |
parents | 8764af78e270 |
children | 87f53c4a13df |
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<tool id="call_consensus" name="5. Call consensus SNPs" version="1.0.1" profile="16.10"> <description>for a sample, or lookup cached consensus SNP calls</description> <requirements> <requirement type="package" version="1.0.6">bzip2</requirement> <requirement type="package" version="1.0.1">snp-pipeline</requirement> <requirement type="package" version="1.5.11">boto3</requirement> <requirement type="package" version="3.4">python3</requirement> </requirements> <command detect_errors="exit_code"><![CDATA[ #set $identifier = $call_pileup.name.split(' ')[0] python $__tool_directory__/snp-cache.py snp_consenus_calls "\$(md5sum $snps $calls $call_pileup | cut -c -32 | md5sum | cut -c -32)" -c " mkdir ./$identifier/ && cp $call_pileup ./$identifier/reads.all.pileup && cfsan_snp_pipeline call_consensus -v 4 -l $snps --vcfFileName "consensus.vcf" ./$identifier/reads.all.pileup 1>&2 && tar zcv ./consensus.vcf ./consensus.fasta " -l $cache_log | tar zvx ./ && cp ./consensus.fasta $calls_consensus_fa && cp ./consensus.vcf $calls_consensus_vcf && cat $cache_log ]]></command> <inputs> <param type="data" name="snps" format="txt" /> <param type="data" name="calls" format="vcf" /> <param type="data" name="call_pileup" format="pileup" /> </inputs> <outputs> <data label="${calls.name.split(' ')[0]} consensus SNPs (VCF)" name="calls_consensus_vcf" format="vcf" /> <data label="${calls.name.split(' ')[0]} consensus SNPs (FASTA)" name="calls_consensus_fa" format="fasta" hidden="true"/> <data label="S3 Cache log" name="cache_log" format="txt" hidden="true" /> </outputs> <tests> <test> <param name="snps" value="snplist.txt" /> <param name="calls" value="samples/sample1/var.flt.vcf" /> <param name="call_pileup" value="samples/sample1/reads.all.pileup" /> <output name="calls_consensus_vcf" value="samples/sample1/consensus.vcf" lines_diff="6" /> <output name="calls_consensus_fa" value="samples/sample1/consensus.fasta" lines_diff="2" /> </test> </tests> <help><![CDATA[ <a href="http://snp-pipeline.readthedocs.io/en/latest/index.html">http://snp-pipeline.readthedocs.io/en/latest/index.html</a> ]]></help> <citations> <citation type="doi">10.7717/peerj-cs.20</citation> </citations> </tool>