Mercurial > repos > kkonganti > cfsan_bettercallsal
comparison 0.5.0/modules/fastp/main.nf @ 1:365849f031fd
"planemo upload"
author | kkonganti |
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date | Mon, 05 Jun 2023 18:48:51 -0400 |
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0:a4b1ee4b68b1 | 1:365849f031fd |
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1 process FASTP { | |
2 tag "$meta.id" | |
3 label 'process_low' | |
4 | |
5 module (params.enable_module ? "${params.swmodulepath}${params.fs}fastp${params.fs}0.23.2" : null) | |
6 conda (params.enable_conda ? "bioconda::fastp=0.23.2 conda-forge::isa-l" : null) | |
7 container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? | |
8 'https://depot.galaxyproject.org/singularity/fastp:0.23.2--h79da9fb_0' : | |
9 'quay.io/biocontainers/fastp:0.23.2--h79da9fb_0' }" | |
10 | |
11 input: | |
12 tuple val(meta), path(reads) | |
13 | |
14 output: | |
15 tuple val(meta), path('*.fastp.fastq.gz') , emit: passed_reads, optional: true | |
16 tuple val(meta), path('*.fail.fastq.gz') , emit: failed_reads, optional: true | |
17 tuple val(meta), path('*.merged.fastq.gz'), emit: merged_reads, optional: true | |
18 tuple val(meta), path('*.json') , emit: json | |
19 tuple val(meta), path('*.html') , emit: html | |
20 tuple val(meta), path('*.log') , emit: log | |
21 path "versions.yml" , emit: versions | |
22 | |
23 when: | |
24 task.ext.when == null || task.ext.when | |
25 | |
26 script: | |
27 def args = task.ext.args ?: '' | |
28 def prefix = task.ext.prefix ?: "${meta.id}" | |
29 def fail_fastq = params.fastp_failed_out && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : params.fastp_failed_out && !meta.single_end ? "--unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : '' | |
30 // Added soft-links to original fastqs for consistent naming in MultiQC | |
31 // Use single ended for interleaved. Add --interleaved_in in config. | |
32 if ( task.ext.args?.contains('--interleaved_in') ) { | |
33 """ | |
34 [ ! -f ${prefix}.fastq.gz ] && ln -sf $reads ${prefix}.fastq.gz | |
35 | |
36 fastp \\ | |
37 --stdout \\ | |
38 --in1 ${prefix}.fastq.gz \\ | |
39 --thread $task.cpus \\ | |
40 --json ${prefix}.fastp.json \\ | |
41 --html ${prefix}.fastp.html \\ | |
42 $fail_fastq \\ | |
43 $args \\ | |
44 2> ${prefix}.fastp.log \\ | |
45 | gzip -c > ${prefix}.fastp.fastq.gz | |
46 | |
47 cat <<-END_VERSIONS > versions.yml | |
48 "${task.process}": | |
49 fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") | |
50 END_VERSIONS | |
51 """ | |
52 } else if (meta.single_end) { | |
53 """ | |
54 [ ! -f ${prefix}.fastq.gz ] && ln -sf $reads ${prefix}.fastq.gz | |
55 | |
56 fastp \\ | |
57 --in1 ${prefix}.fastq.gz \\ | |
58 --out1 ${prefix}.fastp.fastq.gz \\ | |
59 --thread $task.cpus \\ | |
60 --json ${prefix}.fastp.json \\ | |
61 --html ${prefix}.fastp.html \\ | |
62 $fail_fastq \\ | |
63 $args \\ | |
64 2> ${prefix}.fastp.log | |
65 | |
66 cat <<-END_VERSIONS > versions.yml | |
67 "${task.process}": | |
68 fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") | |
69 END_VERSIONS | |
70 """ | |
71 } else { | |
72 def merge_fastq = params.fastp_merged_out ? "-m --merged_out ${prefix}.merged.fastq.gz" : '' | |
73 """ | |
74 [ ! -f ${prefix}_1.fastq.gz ] && ln -sf ${reads[0]} ${prefix}_1.fastq.gz | |
75 [ ! -f ${prefix}_2.fastq.gz ] && ln -sf ${reads[1]} ${prefix}_2.fastq.gz | |
76 fastp \\ | |
77 --in1 ${prefix}_1.fastq.gz \\ | |
78 --in2 ${prefix}_2.fastq.gz \\ | |
79 --out1 ${prefix}_1.fastp.fastq.gz \\ | |
80 --out2 ${prefix}_2.fastp.fastq.gz \\ | |
81 --json ${prefix}.fastp.json \\ | |
82 --html ${prefix}.fastp.html \\ | |
83 $fail_fastq \\ | |
84 $merge_fastq \\ | |
85 --thread $task.cpus \\ | |
86 --detect_adapter_for_pe \\ | |
87 $args \\ | |
88 2> ${prefix}.fastp.log | |
89 | |
90 cat <<-END_VERSIONS > versions.yml | |
91 "${task.process}": | |
92 fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g") | |
93 END_VERSIONS | |
94 """ | |
95 } | |
96 } |