Mercurial > repos > kkonganti > cfsan_bettercallsal
diff 0.5.0/lib/help/fastp.nf @ 1:365849f031fd
"planemo upload"
author | kkonganti |
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date | Mon, 05 Jun 2023 18:48:51 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/0.5.0/lib/help/fastp.nf Mon Jun 05 18:48:51 2023 -0400 @@ -0,0 +1,280 @@ +// Help text for fastp within CPIPES. + +def fastpHelp(params) { + + Map tool = [:] + Map toolspecs = [:] + tool.text = [:] + tool.helpparams = [:] + + toolspecs = [ + 'fastp_run': [ + clihelp: 'Run fastp tool. Default: ' + + (params.fastp_run ?: false), + cliflag: null, + clivalue: null + ], + 'fastp_failed_out': [ + clihelp: 'Specify whether to store reads that cannot pass the filters. ' + + "Default: ${params.fastp_failed_out}", + cliflag: null, + clivalue: null + ], + 'fastp_merged_out': [ + clihelp: 'Specify whether to store merged output or not. ' + + "Default: ${params.fastp_merged_out}", + cliflag: null, + clivalue: null + ], + 'fastp_overlapped_out': [ + clihelp: 'For each read pair, output the overlapped region if it has no mismatched base. ' + + "Default: ${params.fastp_overlapped_out}", + cliflag: '--overlapped_out', + clivalue: (params.fastp_overlapped_out ?: '') + ], + 'fastp_6': [ + clihelp: "Indicate that the input is using phred64 scoring (it'll be converted to phred33, " + + 'so the output will still be phred33). ' + + "Default: ${params.fastp_6}", + cliflag: '-6', + clivalue: (params.fastp_6 ? ' ' : '') + ], + 'fastp_reads_to_process': [ + clihelp: 'Specify how many reads/pairs are to be processed. Default value 0 means ' + + 'process all reads. ' + + "Default: ${params.fastp_reads_to_process}", + cliflag: '--reads_to_process', + clivalue: (params.fastp_reads_to_process ?: '') + ], + 'fastp_fix_mgi_id': [ + clihelp: 'The MGI FASTQ ID format is not compatible with many BAM operation tools, ' + + 'enable this option to fix it. ' + + "Default: ${params.fastp_fix_mgi_id}", + cliflag: '--fix_mgi_id', + clivalue: (params.fastp_fix_mgi_id ? ' ' : '') + ], + 'fastp_A': [ + clihelp: 'Disable adapter trimming. On by default. ' + + "Default: ${params.fastp_A}", + cliflag: '-A', + clivalue: (params.fastp_A ? ' ' : '') + ], + 'fastp_adapter_fasta': [ + clihelp: 'Specify a FASTA file to trim both read1 and read2 (if PE) by all the sequences ' + + 'in this FASTA file. ' + + "Default: ${params.fastp_adapter_fasta}", + cliflag: '--adapter_fasta', + clivalue: (params.fastp_adapter_fasta ?: '') + ], + 'fastp_f': [ + clihelp: 'Trim how many bases in front of read1. ' + + "Default: ${params.fastp_f}", + cliflag: '-f', + clivalue: (params.fastp_f ?: '') + ], + 'fastp_t': [ + clihelp: 'Trim how many bases at the end of read1. ' + + "Default: ${params.fastp_t}", + cliflag: '-t', + clivalue: (params.fastp_t ?: '') + ], + 'fastp_b': [ + clihelp: 'Max length of read1 after trimming. ' + + "Default: ${params.fastp_b}", + cliflag: '-b', + clivalue: (params.fastp_b ?: '') + ], + 'fastp_F': [ + clihelp: 'Trim how many bases in front of read2. ' + + "Default: ${params.fastp_F}", + cliflag: '-F', + clivalue: (params.fastp_F ?: '') + ], + 'fastp_T': [ + clihelp: 'Trim how many bases at the end of read2. ' + + "Default: ${params.fastp_T}", + cliflag: '-T', + clivalue: (params.fastp_T ?: '') + ], + 'fastp_B': [ + clihelp: 'Max length of read2 after trimming. ' + + "Default: ${params.fastp_B}", + cliflag: '-B', + clivalue: (params.fastp_B ?: '') + ], + 'fastp_dedup': [ + clihelp: 'Enable deduplication to drop the duplicated reads/pairs. ' + + "Default: ${params.fastp_dedup}", + cliflag: '--dedup', + clivalue: (params.fastp_dedup ? ' ' : '') + ], + 'fastp_dup_calc_accuracy': [ + clihelp: 'Accuracy level to calculate duplication (1~6), higher level uses more memory ' + + '(1G, 2G, 4G, 8G, 16G, 24G). Default 1 for no-dedup mode, and 3 for dedup mode. ' + + "Default: ${params.fastp_dup_calc_accuracy}", + cliflag: '--dup_calc_accuracy', + clivalue: (params.fastp_dup_calc_accuracy ?: '') + ], + 'fastp_poly_g_min_len': [ + clihelp: 'The minimum length to detect polyG in the read tail. ' + + "Default: ${params.fastp_poly_g_min_len}", + cliflag: '--poly_g_min_len', + clivalue: (params.fastp_poly_g_min_len ?: '') + ], + 'fastp_G': [ + clihelp: 'Disable polyG tail trimming. ' + + "Default: ${params.fastp_G}", + cliflag: '-G', + clivalue: (params.fastp_G ? ' ' : '') + ], + 'fastp_x': [ + clihelp: "Enable polyX trimming in 3' ends. " + + "Default: ${params.fastp_x}", + cliflag: 'x=', + clivalue: (params.fastp_x ? ' ' : '') + ], + 'fastp_poly_x_min_len': [ + clihelp: 'The minimum length to detect polyX in the read tail. ' + + "Default: ${params.fastp_poly_x_min_len}", + cliflag: '--poly_x_min_len', + clivalue: (params.fastp_poly_x_min_len ?: '') + ], + 'fastp_cut_front': [ + clihelp: "Move a sliding window from front (5') to tail, drop the bases in the window " + + 'if its mean quality < threshold, stop otherwise. ' + + "Default: ${params.fastp_cut_front}", + cliflag: '--cut_front', + clivalue: (params.fastp_cut_front ? ' ' : '') + ], + 'fastp_cut_tail': [ + clihelp: "Move a sliding window from tail (3') to front, drop the bases in the window " + + 'if its mean quality < threshold, stop otherwise. ' + + "Default: ${params.fastp_cut_tail}", + cliflag: '--cut_tail', + clivalue: (params.fastp_cut_tail ? ' ' : '') + ], + 'fastp_cut_right': [ + clihelp: "Move a sliding window from tail, drop the bases in the window and the right part " + + 'if its mean quality < threshold, and then stop. ' + + "Default: ${params.fastp_cut_right}", + cliflag: '--cut_right', + clivalue: (params.fastp_cut_right ? ' ' : '') + ], + 'fastp_W': [ + clihelp: "Sliding window size shared by --fastp_cut_front, --fastp_cut_tail and " + + '--fastp_cut_right. ' + + "Default: ${params.fastp_W}", + cliflag: '--cut_window_size', + clivalue: (params.fastp_W ?: '') + ], + 'fastp_M': [ + clihelp: "The mean quality requirement shared by --fastp_cut_front, --fastp_cut_tail and " + + '--fastp_cut_right. ' + + "Default: ${params.fastp_M}", + cliflag: '--cut_mean_quality', + clivalue: (params.fastp_M ?: '') + ], + 'fastp_q': [ + clihelp: 'The quality value below which a base should is not qualified. ' + + "Default: ${params.fastp_q}", + cliflag: '-q', + clivalue: (params.fastp_q ?: '') + ], + 'fastp_u': [ + clihelp: 'What percent of bases are allowed to be unqualified. ' + + "Default: ${params.fastp_u}", + cliflag: '-u', + clivalue: (params.fastp_u ?: '') + ], + 'fastp_n': [ + clihelp: "How many N's can a read have. " + + "Default: ${params.fastp_n}", + cliflag: '-n', + clivalue: (params.fastp_n ?: '') + ], + 'fastp_e': [ + clihelp: "If the full reads' average quality is below this value, then it is discarded. " + + "Default: ${params.fastp_e}", + cliflag: '-e', + clivalue: (params.fastp_e ?: '') + ], + 'fastp_l': [ + clihelp: 'Reads shorter than this length will be discarded. ' + + "Default: ${params.fastp_l}", + cliflag: '-l', + clivalue: (params.fastp_l ?: '') + ], + 'fastp_max_len': [ + clihelp: 'Reads longer than this length will be discarded. ' + + "Default: ${params.fastp_max_len}", + cliflag: '--length_limit', + clivalue: (params.fastp_max_len ?: '') + ], + 'fastp_y': [ + clihelp: 'Enable low complexity filter. The complexity is defined as the percentage ' + + 'of bases that are different from its next base (base[i] != base[i+1]). ' + + "Default: ${params.fastp_y}", + cliflag: '-y', + clivalue: (params.fastp_y ? ' ' : '') + ], + 'fastp_Y': [ + clihelp: 'The threshold for low complexity filter (0~100). Ex: A value of 30 means ' + + '30% complexity is required. ' + + "Default: ${params.fastp_Y}", + cliflag: '-Y', + clivalue: (params.fastp_Y ?: '') + ], + 'fastp_U': [ + clihelp: 'Enable Unique Molecular Identifier (UMI) pre-processing. ' + + "Default: ${params.fastp_U}", + cliflag: '-U', + clivalue: (params.fastp_U ? ' ' : '') + ], + 'fastp_umi_loc': [ + clihelp: 'Specify the location of UMI, can be one of ' + + 'index1/index2/read1/read2/per_index/per_read. ' + + "Default: ${params.fastp_umi_loc}", + cliflag: '--umi_loc', + clivalue: (params.fastp_umi_loc ?: '') + ], + 'fastp_umi_len': [ + clihelp: 'If the UMI is in read1 or read2, its length should be provided. ' + + "Default: ${params.fastp_umi_len}", + cliflag: '--umi_len', + clivalue: (params.fastp_umi_len ?: '') + ], + 'fastp_umi_prefix': [ + clihelp: 'If specified, an underline will be used to connect prefix and UMI ' + + '(i.e. prefix=UMI, UMI=AATTCG, final=UMI_AATTCG). ' + + "Default: ${params.fastp_umi_prefix}", + cliflag: '--umi_prefix', + clivalue: (params.fastp_umi_prefix ?: '') + ], + 'fastp_umi_skip': [ + clihelp: 'If the UMI is in read1 or read2, fastp can skip several bases following the UMI. ' + + "Default: ${params.fastp_umi_skip}", + cliflag: '--umi_skip', + clivalue: (params.fastp_umi_skip ?: '') + ], + 'fastp_p': [ + clihelp: 'Enable overrepresented sequence analysis. ' + + "Default: ${params.fastp_p}", + cliflag: '-p', + clivalue: (params.fastp_p ? ' ' : '') + ], + 'fastp_P': [ + clihelp: 'One in this many number of reads will be computed for overrepresentation analysis ' + + '(1~10000), smaller is slower. ' + + "Default: ${params.fastp_P}", + cliflag: '-P', + clivalue: (params.fastp_P ?: '') + ] + ] + + toolspecs.each { + k, v -> tool.text['--' + k] = "${v.clihelp}" + tool.helpparams[k] = [ cliflag: "${v.cliflag}", clivalue: v.clivalue ] + } + + return tool +} \ No newline at end of file