Mercurial > repos > kkonganti > cfsan_bettercallsal
view 0.5.0/modules/salmon/quant/main.nf @ 6:83ed52d5af32
"planemo upload"
author | kkonganti |
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date | Tue, 06 Jun 2023 10:05:24 -0400 |
parents | 365849f031fd |
children | a492d1511fb6 |
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process SALMON_QUANT { tag "$meta.id" label "process_medium" module (params.enable_module ? "${params.swmodulepath}${params.fs}salmon${params.fs}1.9.0" : null) conda (params.enable_conda ? 'conda-forge::libgcc-ng bioconda::salmon=1.9.0' : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/salmon:1.9.0--h7e5ed60_1' : 'quay.io/biocontainers/salmon:1.9.0--h7e5ed60_1' }" input: tuple val(meta), path(reads_or_bam), path(index_or_tr_fasta) output: tuple val(meta), path("${meta.id}_salmon_res"), emit: results path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}_salmon_res" def reference = "--index $index_or_tr_fasta" def lib_type = (meta.salmon_lib_type ?: '') def alignment_mode = (meta.salmon_alignment_mode ?: '') def gtf = (meta.salmon_gtf ? "--geneMap ${meta.salmon_gtf}" : '') def input_reads =(meta.single_end ? "-r $reads_or_bam" : "-1 ${reads_or_bam[0]} -2 ${reads_or_bam[1]}") // Use path(reads_or_bam) to point to BAM and path(index_or_tr_fasta) to point to transcript fasta // if using salmon DSL2 module in alignment-based mode. // By default, this module will be run in selective-alignment-based mode of salmon. if (alignment_mode) { reference = "-t $index_or_tr_fasta" input_reads = "-a $reads_or_bam" } def strandedness_opts = [ 'A', 'U', 'SF', 'SR', 'IS', 'IU' , 'ISF', 'ISR', 'OS', 'OU' , 'OSF', 'OSR', 'MS', 'MU' , 'MSF', 'MSR' ] def strandedness = 'A' if (lib_type) { if (strandedness_opts.contains(lib_type)) { strandedness = lib_type } else { log.info "[Salmon Quant] Invalid library type specified '--libType=${lib_type}', defaulting to auto-detection with '--libType=A'." } } else { strandedness = meta.single_end ? 'U' : 'IU' if (meta.strandedness == 'forward') { strandedness = meta.single_end ? 'SF' : 'ISF' } else if (meta.strandedness == 'reverse') { strandedness = meta.single_end ? 'SR' : 'ISR' } } """ salmon quant \\ --threads $task.cpus \\ --libType=$strandedness \\ $gtf \\ $args \\ -o $prefix \\ $reference \\ $input_reads cat <<-END_VERSIONS > versions.yml "${task.process}": salmon: \$(echo \$(salmon --version) | sed -e "s/salmon //g") END_VERSIONS """ }