kkonganti@17: // Define any required imports for this specific workflow
kkonganti@17: import java.nio.file.Paths
kkonganti@17: import java.util.zip.GZIPInputStream
kkonganti@17: import java.io.FileInputStream
kkonganti@17: import nextflow.file.FileHelper
kkonganti@17: 
kkonganti@17: 
kkonganti@17: // Include any necessary methods
kkonganti@17: include { \
kkonganti@17:     summaryOfParams; stopNow; fastqEntryPointHelp; sendMail; conciseHelp; \
kkonganti@17:     addPadding; wrapUpHelp     } from "${params.routines}"
kkonganti@17: include { bbmergeHelp          } from "${params.toolshelp}${params.fs}bbmerge"
kkonganti@17: include { fastpHelp            } from "${params.toolshelp}${params.fs}fastp"
kkonganti@17: include { mashscreenHelp       } from "${params.toolshelp}${params.fs}mashscreen"
kkonganti@17: include { tuspyHelp            } from "${params.toolshelp}${params.fs}tuspy"
kkonganti@17: include { sourmashsketchHelp   } from "${params.toolshelp}${params.fs}sourmashsketch"
kkonganti@17: include { sourmashgatherHelp   } from "${params.toolshelp}${params.fs}sourmashgather"
kkonganti@17: include { sourmashsearchHelp   } from "${params.toolshelp}${params.fs}sourmashsearch"
kkonganti@17: include { sfhpyHelp            } from "${params.toolshelp}${params.fs}sfhpy"
kkonganti@17: include { kmaindexHelp         } from "${params.toolshelp}${params.fs}kmaindex"
kkonganti@17: include { kmaalignHelp         } from "${params.toolshelp}${params.fs}kmaalign"
kkonganti@17: include { megahitHelp          } from "${params.toolshelp}${params.fs}megahit"
kkonganti@17: include { mlstHelp             } from "${params.toolshelp}${params.fs}mlst"
kkonganti@17: include { abricateHelp         } from "${params.toolshelp}${params.fs}abricate"
kkonganti@17: include { salmonidxHelp        } from "${params.toolshelp}${params.fs}salmonidx"
kkonganti@17: include { gsrpyHelp            } from "${params.toolshelp}${params.fs}gsrpy"
kkonganti@17: 
kkonganti@17: // Exit if help requested before any subworkflows
kkonganti@17: if (params.help) {
kkonganti@17:     log.info help()
kkonganti@17:     exit 0
kkonganti@17: }
kkonganti@17: 
kkonganti@17: 
kkonganti@17: // Include any necessary modules and subworkflows
kkonganti@17: include { PROCESS_FASTQ           } from "${params.subworkflows}${params.fs}process_fastq"
kkonganti@17: include { CAT_CAT                 } from "${params.modules}${params.fs}cat_cat${params.fs}main"
kkonganti@17: include { FASTQC                  } from "${params.modules}${params.fs}fastqc${params.fs}main"
kkonganti@17: include { BBTOOLS_BBMERGE         } from "${params.modules}${params.fs}bbtools${params.fs}bbmerge${params.fs}main"
kkonganti@17: include { FASTP                   } from "${params.modules}${params.fs}fastp${params.fs}main"
kkonganti@17: include { MASH_SCREEN             } from "${params.modules}${params.fs}mash${params.fs}screen${params.fs}main"
kkonganti@17: include { TOP_UNIQUE_SEROVARS     } from "${params.modules}${params.fs}top_unique_serovars${params.fs}main"
kkonganti@17: include { SOURMASH_SKETCH         } from "${params.modules}${params.fs}sourmash${params.fs}sketch${params.fs}main"
kkonganti@17: include { SOURMASH_GATHER         } from "${params.modules}${params.fs}sourmash${params.fs}gather${params.fs}main"
kkonganti@17: include { SOURMASH_SEARCH         } from "${params.modules}${params.fs}sourmash${params.fs}search${params.fs}main"
kkonganti@17: include { KMA_INDEX               } from "${params.modules}${params.fs}kma${params.fs}index${params.fs}main"
kkonganti@17: include { KMA_ALIGN               } from "${params.modules}${params.fs}kma${params.fs}align${params.fs}main"
kkonganti@17: include { OTF_GENOME              } from "${params.modules}${params.fs}otf_genome${params.fs}main"
kkonganti@17: include { MEGAHIT_ASSEMBLE        } from "${params.modules}${params.fs}megahit${params.fs}assemble${params.fs}main"
kkonganti@17: include { MLST                    } from "${params.modules}${params.fs}mlst${params.fs}main"
kkonganti@17: include { ABRICATE_RUN            } from "${params.modules}${params.fs}abricate${params.fs}run${params.fs}main"
kkonganti@17: include { ABRICATE_SUMMARY        } from "${params.modules}${params.fs}abricate${params.fs}summary${params.fs}main"
kkonganti@17: include { TABLE_SUMMARY           } from "${params.modules}${params.fs}cat${params.fs}tables${params.fs}main"
kkonganti@17: include { SALMON_INDEX            } from "${params.modules}${params.fs}salmon${params.fs}index${params.fs}main"
kkonganti@17: include { SALMON_QUANT            } from "${params.modules}${params.fs}salmon${params.fs}quant${params.fs}main"
kkonganti@17: include { SOURMASH_COMPARE        } from "${params.modules}${params.fs}custom${params.fs}sourmash${params.fs}compare${params.fs}main"
kkonganti@17: include { BCS_DISTANCE_MATRIX     } from "${params.modules}${params.fs}bcs_distance_matrix${params.fs}main"
kkonganti@17: include { BCS_RESULTS             } from "${params.modules}${params.fs}bcs_results${params.fs}main"
kkonganti@17: include { DUMP_SOFTWARE_VERSIONS  } from "${params.modules}${params.fs}custom${params.fs}dump_software_versions${params.fs}main"
kkonganti@17: include { MULTIQC                 } from "${params.modules}${params.fs}multiqc${params.fs}main"
kkonganti@17: 
kkonganti@17: /*
kkonganti@17: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
kkonganti@17:     INPUTS AND ANY CHECKS FOR THE BETTERCALLSAL WORKFLOW
kkonganti@17: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
kkonganti@17: */
kkonganti@17: 
kkonganti@17: def reads_platform = 0
kkonganti@17: def salmon_idx_decoys = file ( "${params.salmonidx_decoys}" )
kkonganti@17: def abricate_dbs = [ 'ncbiamrplus', 'resfinder', 'megares', 'argannot' ]
kkonganti@17: 
kkonganti@17: reads_platform += (params.input ? 1 : 0)
kkonganti@17: 
kkonganti@17: if (reads_platform < 1 || reads_platform == 0) {
kkonganti@17:     stopNow("Please mention at least one absolute path to input folder which contains\n" +
kkonganti@17:             "FASTQ files sequenced using the --input option.\n" +
kkonganti@17:         "Ex: --input (Illumina or Generic short reads in FASTQ format)")
kkonganti@17: }
kkonganti@17: 
kkonganti@17: checkMetadataExists(params.mash_sketch, 'MASH sketch')
kkonganti@17: checkMetadataExists(params.tuspy_ps, 'ACC2SERO pickle')
kkonganti@17: checkMetadataExists(params.gsrpy_snp_clus_metadata, 'PDG reference target cluster metadata')
kkonganti@17: 
kkonganti@17: /*
kkonganti@17: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
kkonganti@17:     RUN THE BETTERCALLSAL WORKFLOW
kkonganti@17: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
kkonganti@17: */
kkonganti@17: 
kkonganti@17: workflow BETTERCALLSAL {
kkonganti@17:     main:
kkonganti@17:         log.info summaryOfParams()
kkonganti@17: 
kkonganti@17:         PROCESS_FASTQ()
kkonganti@17: 
kkonganti@17:         PROCESS_FASTQ
kkonganti@17:             .out
kkonganti@17:             .versions
kkonganti@17:             .set { software_versions }
kkonganti@17: 
kkonganti@17:         PROCESS_FASTQ
kkonganti@17:             .out
kkonganti@17:             .processed_reads
kkonganti@17:             .set { ch_processed_reads }
kkonganti@17: 
kkonganti@17:         if (params.bbmerge_run && !params.fq_single_end) {
kkonganti@17:             ch_processed_reads
kkonganti@17:                 .map { meta, fastq ->
kkonganti@17:                     meta.adapters = (params.bbmerge_adapters ?: params.dummyfile)
kkonganti@17:                     [ meta, fastq ]
kkonganti@17:                 }
kkonganti@17:                 .set { ch_processed_reads }
kkonganti@17:             
kkonganti@17:             BBTOOLS_BBMERGE( ch_processed_reads )
kkonganti@17: 
kkonganti@17:             BBTOOLS_BBMERGE
kkonganti@17:                 .out
kkonganti@17:                 .fastq
kkonganti@17:                 .map { meta, fastq -> [ meta, [ fastq ] ] }
kkonganti@17:                 .set { ch_processed_reads }
kkonganti@17: 
kkonganti@17:             software_versions
kkonganti@17:                 .mix ( BBTOOLS_BBMERGE.out.versions )
kkonganti@17:                 .set { software_versions }
kkonganti@17:         }
kkonganti@17: 
kkonganti@17:         if (params.fastp_run) {
kkonganti@17:             FASTP ( ch_processed_reads )
kkonganti@17: 
kkonganti@17:             FASTP
kkonganti@17:                 .out
kkonganti@17:                 .passed_reads
kkonganti@17:                 .set { ch_processed_reads }
kkonganti@17: 
kkonganti@17:             FASTP
kkonganti@17:                 .out
kkonganti@17:                 .json
kkonganti@17:                 .map { meta, json -> [ json ] }
kkonganti@17:                 .collect()
kkonganti@17:                 .set { ch_multiqc }
kkonganti@17: 
kkonganti@17:             software_versions
kkonganti@17:                 .mix ( FASTP.out.versions )
kkonganti@17:                 .set { software_versions }
kkonganti@17:         } else {
kkonganti@17:             FASTQC ( ch_processed_reads )
kkonganti@17: 
kkonganti@17:             FASTQC
kkonganti@17:                 .out
kkonganti@17:                 .zip
kkonganti@17:                 .map { meta, zip -> [ zip ] }
kkonganti@17:                 .collect()
kkonganti@17:                 .set { ch_multiqc }
kkonganti@17: 
kkonganti@17:             software_versions
kkonganti@17:                 .mix ( FASTQC.out.versions )
kkonganti@17:                 .set { software_versions }
kkonganti@17:         }
kkonganti@17: 
kkonganti@17:         if (params.bcs_concat_pe && !params.fq_single_end && !params.bbmerge_run) {
kkonganti@17:             CAT_CAT ( ch_processed_reads )
kkonganti@17: 
kkonganti@17:             CAT_CAT
kkonganti@17:                 .out
kkonganti@17:                 .concatenated_reads
kkonganti@17:                 .set { ch_processed_reads }
kkonganti@17: 
kkonganti@17:             software_versions
kkonganti@17:                 .mix ( CAT_CAT.out.versions )
kkonganti@17:                 .set { software_versions }
kkonganti@17:         }
kkonganti@17: 
kkonganti@17:         ch_processed_reads
kkonganti@17:             .map { meta, fastq ->
kkonganti@17:                 meta.sequence_sketch = params.mash_sketch
kkonganti@17:                 meta.get_kma_hit_accs = true
kkonganti@17:                 meta.single_end = true
kkonganti@17:                 meta.salmon_decoys = params.dummyfile
kkonganti@17:                 meta.salmon_lib_type = (params.salmonalign_libtype ?: false)
kkonganti@17:                 [ meta, fastq ]
kkonganti@17:             }
kkonganti@17:             .filter { meta, fastq ->
kkonganti@17:                 fq_file = ( fastq.getClass().toString() =~ /ArrayList/ ? fastq : [ fastq ] )
kkonganti@17:                 fq_gzip = new GZIPInputStream( new FileInputStream( fq_file[0].toString() ) )
kkonganti@17:                 fq_gzip.read() != -1
kkonganti@17:             }
kkonganti@17:             .set { ch_processed_reads }
kkonganti@17: 
kkonganti@17:         MASH_SCREEN ( ch_processed_reads )
kkonganti@17: 
kkonganti@17:         TOP_UNIQUE_SEROVARS ( MASH_SCREEN.out.screened )
kkonganti@17:         
kkonganti@17:         TOP_UNIQUE_SEROVARS.out.genomes_fasta
kkonganti@17:             .set { ch_genomes_fasta }
kkonganti@17: 
kkonganti@17:         TOP_UNIQUE_SEROVARS.out.failed
kkonganti@17:             .set { ch_bcs_calls_failed }
kkonganti@17: 
kkonganti@17:         if (params.sourmashgather_run || params.sourmashsearch_run) {
kkonganti@17:             SOURMASH_SKETCH (
kkonganti@17:                 ch_processed_reads
kkonganti@17:                     .join ( ch_genomes_fasta )
kkonganti@17:             )
kkonganti@17: 
kkonganti@17:             if (params.sourmashgather_run) {
kkonganti@17:                 SOURMASH_GATHER (
kkonganti@17:                     SOURMASH_SKETCH.out.signatures,
kkonganti@17:                     [], [], [], []
kkonganti@17:                 )
kkonganti@17: 
kkonganti@17:                 SOURMASH_GATHER
kkonganti@17:                     .out
kkonganti@17:                     .genomes_fasta
kkonganti@17:                     .set { ch_genomes_fasta }
kkonganti@17: 
kkonganti@17:                 ch_bcs_calls_failed
kkonganti@17:                     .concat ( SOURMASH_GATHER.out.failed )
kkonganti@17:                     .set { ch_bcs_calls_failed }
kkonganti@17: 
kkonganti@17:                 software_versions
kkonganti@17:                     .mix ( SOURMASH_GATHER.out.versions.ifEmpty(null) )
kkonganti@17:                     .set { software_versions }
kkonganti@17:             }
kkonganti@17: 
kkonganti@17:             if (params.sourmashsearch_run) {
kkonganti@17:                 SOURMASH_SEARCH (
kkonganti@17:                     SOURMASH_SKETCH.out.signatures,
kkonganti@17:                     []
kkonganti@17:                 )
kkonganti@17: 
kkonganti@17:                 SOURMASH_SEARCH
kkonganti@17:                     .out
kkonganti@17:                     .genomes_fasta
kkonganti@17:                     .set { ch_genomes_fasta }
kkonganti@17: 
kkonganti@17:                 ch_bcs_calls_failed
kkonganti@17:                     .concat ( SOURMASH_SEARCH.out.failed )
kkonganti@17:                     .set { ch_bcs_calls_failed }
kkonganti@17: 
kkonganti@17:                 software_versions
kkonganti@17:                     .mix ( SOURMASH_SEARCH.out.versions.ifEmpty(null) )
kkonganti@17:                     .set { software_versions }
kkonganti@17:             }
kkonganti@17:         }
kkonganti@17: 
kkonganti@17:         KMA_INDEX ( ch_genomes_fasta )
kkonganti@17: 
kkonganti@17:         KMA_ALIGN ( 
kkonganti@17:             ch_processed_reads
kkonganti@17:                 .join ( KMA_INDEX.out.idx )
kkonganti@17:         )
kkonganti@17: 
kkonganti@17:         OTF_GENOME (
kkonganti@17:             KMA_ALIGN.out.hits
kkonganti@17:                 .join ( KMA_ALIGN.out.frags )
kkonganti@17:         )
kkonganti@17: 
kkonganti@17:         OTF_GENOME.out.failed
kkonganti@17:             .concat ( ch_bcs_calls_failed )
kkonganti@17:             .collectFile( name: 'BCS_NO_CALLS.txt' )
kkonganti@17:             .set { ch_bcs_no_calls }
kkonganti@17: 
kkonganti@17:         SALMON_INDEX ( OTF_GENOME.out.genomes_fasta )
kkonganti@17: 
kkonganti@17:         SALMON_QUANT (
kkonganti@17:             ch_processed_reads
kkonganti@17:                 .join ( SALMON_INDEX.out.idx )
kkonganti@17:         )
kkonganti@17: 
kkonganti@17:         SALMON_QUANT
kkonganti@17:             .out
kkonganti@17:             .results
kkonganti@17:             .groupTuple(by: [0])
kkonganti@17:             .map { it -> tuple ( it[1].flatten() ) }
kkonganti@17:             .mix ( ch_bcs_no_calls )
kkonganti@17:             .collect()
kkonganti@17:             .set { ch_salmon_res_dirs }
kkonganti@17: 
kkonganti@17:         if (params.sourmashsketch_run) {
kkonganti@17:             SOURMASH_SKETCH
kkonganti@17:                 .out
kkonganti@17:                 .signatures
kkonganti@17:                 .groupTuple(by: [0])
kkonganti@17:                 .map { meta, qsigs, dsigs -> [ qsigs ] }
kkonganti@17:                 .collect()
kkonganti@17:                 .flatten()
kkonganti@17:                 .collect()
kkonganti@17:                 .set { ch_query_sigs }
kkonganti@17: 
kkonganti@17:             KMA_ALIGN
kkonganti@17:                 .out
kkonganti@17:                 .hits
kkonganti@17:                 .map { meta, hits -> [ hits ] }
kkonganti@17:                 .collect()
kkonganti@17:                 .flatten()
kkonganti@17:                 .collectFile(name: 'accessions.txt')
kkonganti@17:                 .set { ch_otf_genomes }
kkonganti@17: 
kkonganti@17:             if (params.megahit_run) {
kkonganti@17: 
kkonganti@17:                 MEGAHIT_ASSEMBLE ( OTF_GENOME.out.reads_extracted )
kkonganti@17: 
kkonganti@17:                 MEGAHIT_ASSEMBLE
kkonganti@17:                     .out
kkonganti@17:                     .assembly
kkonganti@17:                     .set { ch_asm_filtered_contigs }
kkonganti@17: 
kkonganti@17:                 MLST ( ch_asm_filtered_contigs )
kkonganti@17: 
kkonganti@17:                 MLST.out.tsv
kkonganti@17:                     .map { meta, tsv -> [ 'mlst', tsv] }
kkonganti@17:                     .groupTuple(by: [0])
kkonganti@17:                     .map { it -> tuple ( it[0], it[1].flatten() ) }
kkonganti@17:                     .set { ch_mqc_custom_tbl }
kkonganti@17: 
kkonganti@17:                 ABRICATE_RUN ( 
kkonganti@17:                     ch_asm_filtered_contigs, 
kkonganti@17:                     abricate_dbs
kkonganti@17:                 )
kkonganti@17: 
kkonganti@17:                 ABRICATE_RUN
kkonganti@17:                     .out
kkonganti@17:                     .abricated
kkonganti@17:                     .map { meta, abres -> [ abricate_dbs, abres ] }
kkonganti@17:                     .groupTuple(by: [0])
kkonganti@17:                     .map { it -> tuple ( it[0], it[1].flatten() ) }
kkonganti@17:                     .set { ch_abricated }
kkonganti@17: 
kkonganti@17:                 ABRICATE_SUMMARY ( ch_abricated )
kkonganti@17: 
kkonganti@17:                 ch_mqc_custom_tbl
kkonganti@17:                     .concat (
kkonganti@17:                         ABRICATE_SUMMARY.out.ncbiamrplus.map { it -> tuple ( it[0], it[1] )},
kkonganti@17:                         ABRICATE_SUMMARY.out.resfinder.map { it -> tuple ( it[0], it[1] )},
kkonganti@17:                         ABRICATE_SUMMARY.out.megares.map { it -> tuple ( it[0], it[1] )},
kkonganti@17:                         ABRICATE_SUMMARY.out.argannot.map { it -> tuple ( it[0], it[1] )},
kkonganti@17:                     )
kkonganti@17:                     .groupTuple(by: [0])
kkonganti@17:                     .map { it -> [ it[0], it[1].flatten() ]}
kkonganti@17:                     .set { ch_mqc_custom_tbl }
kkonganti@17: 
kkonganti@17:                 TABLE_SUMMARY ( ch_mqc_custom_tbl )
kkonganti@17: 
kkonganti@17:                 ch_multiqc
kkonganti@17:                     .concat ( TABLE_SUMMARY.out.mqc_yml )
kkonganti@17:                     .set { ch_multiqc }
kkonganti@17: 
kkonganti@17:                 software_versions
kkonganti@17:                     .mix (
kkonganti@17:                         MEGAHIT_ASSEMBLE.out.versions.ifEmpty(null),
kkonganti@17:                         MLST.out.versions.ifEmpty(null),
kkonganti@17:                         ABRICATE_RUN.out.versions.ifEmpty(null),
kkonganti@17:                         ABRICATE_SUMMARY.out.versions.ifEmpty(null),
kkonganti@17:                         TABLE_SUMMARY.out.versions.ifEmpty(null)
kkonganti@17:                     )
kkonganti@17:                     .set { software_versions }
kkonganti@17:             }
kkonganti@17: 
kkonganti@17:             SOURMASH_COMPARE ( ch_query_sigs, ch_otf_genomes )
kkonganti@17: 
kkonganti@17:             BCS_DISTANCE_MATRIX (
kkonganti@17:                 SOURMASH_COMPARE.out.matrix,
kkonganti@17:                 SOURMASH_COMPARE.out.labels
kkonganti@17:             )
kkonganti@17: 
kkonganti@17:             ch_multiqc
kkonganti@17:                 .concat ( BCS_DISTANCE_MATRIX.out.mqc_yml )
kkonganti@17:                 .set { ch_multiqc }
kkonganti@17: 
kkonganti@17:             software_versions
kkonganti@17:                 .mix (
kkonganti@17:                     SOURMASH_SKETCH.out.versions.ifEmpty(null),
kkonganti@17:                     SOURMASH_COMPARE.out.versions.ifEmpty(null),
kkonganti@17:                     BCS_DISTANCE_MATRIX.out.versions.ifEmpty(null),
kkonganti@17:                 )
kkonganti@17:                 .set { software_versions }
kkonganti@17:         }
kkonganti@17: 
kkonganti@17:         BCS_RESULTS ( ch_salmon_res_dirs )
kkonganti@17: 
kkonganti@17:         DUMP_SOFTWARE_VERSIONS (
kkonganti@17:             software_versions
kkonganti@17:                 .mix (
kkonganti@17:                     MASH_SCREEN.out.versions,
kkonganti@17:                     TOP_UNIQUE_SEROVARS.out.versions,
kkonganti@17:                     KMA_INDEX.out.versions,
kkonganti@17:                     KMA_ALIGN.out.versions,
kkonganti@17:                     OTF_GENOME.out.versions.ifEmpty(null),
kkonganti@17:                     SALMON_INDEX.out.versions,
kkonganti@17:                     SALMON_QUANT.out.versions,
kkonganti@17:                     BCS_RESULTS.out.versions
kkonganti@17:                 )
kkonganti@17:                 .unique()
kkonganti@17:                 .collectFile(name: 'collected_versions.yml')
kkonganti@17:         )
kkonganti@17: 
kkonganti@17:         if (params.multiqc_run) {
kkonganti@17:             DUMP_SOFTWARE_VERSIONS
kkonganti@17:                 .out
kkonganti@17:                 .mqc_yml
kkonganti@17:                 .concat (
kkonganti@17:                     ch_multiqc,
kkonganti@17:                     BCS_RESULTS.out.mqc_yml,
kkonganti@17:                     BCS_RESULTS.out.mqc_json
kkonganti@17:                 )
kkonganti@17:                 .collect()
kkonganti@17:                 .set { ch_multiqc }
kkonganti@17: 
kkonganti@17:             MULTIQC ( ch_multiqc )
kkonganti@17:         }
kkonganti@17: 
kkonganti@17: }
kkonganti@17: 
kkonganti@17: /*
kkonganti@17: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
kkonganti@17:     ON COMPLETE, SHOW GORY DETAILS OF ALL PARAMS WHICH WILL BE HELPFUL TO DEBUG
kkonganti@17: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
kkonganti@17: */
kkonganti@17: 
kkonganti@17: workflow.onComplete {
kkonganti@17:     if (workflow.success) {
kkonganti@17:         sendMail()
kkonganti@17:     }
kkonganti@17: }
kkonganti@17: 
kkonganti@17: workflow.onError {
kkonganti@17:     sendMail()
kkonganti@17: }
kkonganti@17: 
kkonganti@17: /*
kkonganti@17: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
kkonganti@17:     METHOD TO CHECK METADATA EXISTENCE
kkonganti@17: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
kkonganti@17: */
kkonganti@17: 
kkonganti@17: def checkMetadataExists(file_path, msg) {
kkonganti@17:     file_path_obj = file( file_path )
kkonganti@17: 
kkonganti@17:     if (!file_path_obj.exists() || file_path_obj.size() == 0) {
kkonganti@17:         stopNow("Please check if your ${msg} file\n" +
kkonganti@17:             "[ ${file_path} ]\nexists and is not of size 0.")
kkonganti@17:     }
kkonganti@17: }
kkonganti@17: 
kkonganti@17: /*
kkonganti@17: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
kkonganti@17:     HELP TEXT METHODS FOR BETTERCALLSAL WORKFLOW
kkonganti@17: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
kkonganti@17: */
kkonganti@17: 
kkonganti@17: def help() {
kkonganti@17: 
kkonganti@17:     Map helptext = [:]
kkonganti@17:     Map bcsConcatHelp = [:]
kkonganti@17:     Map fastpAdapterHelp = [:]
kkonganti@17:     Map nH = [:]
kkonganti@17:     def uHelp = (params.help.getClass().toString() =~ /String/ ? params.help.tokenize(',').join(' ') : '')
kkonganti@17: 
kkonganti@17:     Map defaultHelp = [
kkonganti@17:         '--help bbmerge'          : 'Show bbmerge.sh CLI options',
kkonganti@17:         '--help fastp'            : 'Show fastp CLI options',
kkonganti@17:         '--help mash'             : 'Show mash `screen` CLI options',
kkonganti@17:         '--help tuspy'            : 'Show get_top_unique_mash_hit_genomes.py CLI options',
kkonganti@17:         '--help sourmashsketch'   : 'Show sourmash `sketch` CLI options',
kkonganti@17:         '--help sourmashgather'   : 'Show sourmash `gather` CLI options',
kkonganti@17:         '--help sourmashsearch'   : 'Show sourmash `search` CLI options',
kkonganti@17:         '--help sfhpy'            : 'Show sourmash_filter_hits.py CLI options',
kkonganti@17:         '--help kmaindex'         : 'Show kma `index` CLI options',
kkonganti@17:         '--help kmaalign'         : 'Show kma CLI options',
kkonganti@17:         '--help megahit'          : 'Show megahit CLI options',
kkonganti@17:         '--help mlst'             : 'Show mlst CLI options',
kkonganti@17:         '--help abricate'         : 'Show abricate CLI options',
kkonganti@17:         '--help salmon'           : 'Show salmon `index` CLI options',
kkonganti@17:         '--help gsrpy'            : 'Show gen_salmon_res_table.py CLI options\n'
kkonganti@17:     ]
kkonganti@17: 
kkonganti@17:     bcsConcatHelp['--bcs_concat_pe'] = "Concatenate paired-end files. " +
kkonganti@17:         "Default: ${params.bcs_concat_pe}"
kkonganti@17: 
kkonganti@17:     fastpAdapterHelp['--fastp_use_custom_adapaters'] = "Use custom adapter FASTA with fastp on top of " +
kkonganti@17:         "built-in adapter sequence auto-detection. Enabling this option will attempt to find and remove " +
kkonganti@17:         "all possible Illumina adapter and primer sequences but will make the workflow run slow. " +
kkonganti@17:         "Default: ${params.fastp_use_custom_adapters}"
kkonganti@17: 
kkonganti@17:     if (params.help.getClass().toString() =~ /Boolean/ || uHelp.size() == 0) {
kkonganti@17:         println conciseHelp('fastp,mash')
kkonganti@17:         helptext.putAll(defaultHelp)
kkonganti@17:     } else {
kkonganti@17:         params.help.tokenize(',').each { h ->
kkonganti@17:             if (defaultHelp.keySet().findAll{ it =~ /(?i)\b${h}\b/ }.size() == 0) {
kkonganti@17:                 println conciseHelp('fastp,mash')
kkonganti@17:                 stopNow("Tool [ ${h} ] is not a part of ${params.pipeline} pipeline.")
kkonganti@17:             }
kkonganti@17:         }
kkonganti@17: 
kkonganti@17:         helptext.putAll(
kkonganti@17:             fastqEntryPointHelp() +
kkonganti@17:             bcsConcatHelp +
kkonganti@17:             (uHelp =~ /(?i)\bbbmerge/ ? bbmergeHelp(params).text : nH) +
kkonganti@17:             (uHelp =~ /(?i)\bfastp/ ? fastpHelp(params).text + fastpAdapterHelp : nH) +
kkonganti@17:             (uHelp =~ /(?i)\bmash/ ? mashscreenHelp(params).text : nH) +
kkonganti@17:             (uHelp =~ /(?i)\btuspy/ ? tuspyHelp(params).text : nH) +
kkonganti@17:             (uHelp =~ /(?i)\bsourmashsketch/ ? sourmashsketchHelp(params).text : nH) +
kkonganti@17:             (uHelp =~ /(?i)\bsourmashgather/ ? sourmashgatherHelp(params).text : nH) +
kkonganti@17:             (uHelp =~ /(?i)\bsourmashsearch/ ? sourmashsearchHelp(params).text : nH) +
kkonganti@17:             (uHelp =~ /(?i)\bsfhpy/ ? sfhpyHelp(params).text : nH) +
kkonganti@17:             (uHelp =~ /(?i)\bkmaindex/ ? kmaindexHelp(params).text : nH) +
kkonganti@17:             (uHelp =~ /(?i)\bkmaalign/ ? kmaalignHelp(params).text : nH) +
kkonganti@17:             (uHelp =~ /(?i)\bmegahit/ ? megahitHelp(params).text : nH) +
kkonganti@17:             (uHelp =~ /(?i)\bmlst/ ? mlstHelp(params).text : nH) +
kkonganti@17:             (uHelp =~ /(?i)\babricate/ ? abricateHelp(params).text : nH) +
kkonganti@17:             (uHelp =~ /(?i)\bsalmon/ ? salmonidxHelp(params).text : nH) +
kkonganti@17:             (uHelp =~ /(?i)\bgsrpy/ ? gsrpyHelp(params).text : nH) +
kkonganti@17:             wrapUpHelp()
kkonganti@17:         )
kkonganti@17:     }
kkonganti@17: 
kkonganti@17:     return addPadding(helptext)
kkonganti@17: }