kkonganti@101: params { kkonganti@101: workflow_blueprint_by = 'Narjol.Gonzalez-Escalona@fda.hhs.gov' kkonganti@101: workflow_built_by = 'Kranti.Konganti@fda.hhs.gov' kkonganti@104: workflow_version = '0.2.1' kkonganti@101: centrifuge_x = '/tool-local-data/cfsan-centriflaken-db/0/centrifuge/2022-04-12/ab' kkonganti@101: centrifuge_extract_bug = 'Escherichia coli' kkonganti@101: centrifuge_save_aligned = false kkonganti@101: centrifuge_save_unaligned = false kkonganti@101: centrifuge_out_fmt_sam = false kkonganti@101: centrifuge_ignore_quals = false kkonganti@101: kraken2_db = '/tool-local-data/cfsan-centriflaken-db/0/kraken2/standard-210914' kkonganti@101: kraken2_confidence = '0.0' kkonganti@101: kraken2_quick = false kkonganti@101: kraken2_use_mpa_style = false kkonganti@101: kraken2_minimum_base_quality = '0' kkonganti@101: kraken2_report_zero_counts = false kkonganti@101: kraken2_report_minimizer_data = false kkonganti@101: kraken2_use_names = true kkonganti@101: kraken2_extract_bug = params.centrifuge_extract_bug kkonganti@101: flye_pacbio_raw = false kkonganti@101: flye_pacbio_corr = false kkonganti@101: flye_pacbio_hifi = false kkonganti@101: flye_nano_raw = true kkonganti@101: flye_nano_corr = false kkonganti@101: flye_nano_hq = false kkonganti@101: flye_genome_size = (params.centrifuge_extract_bug ==~ /(?i)Salmonella/ ? '5m' : '5.5m') kkonganti@101: flye_polish_iter = false kkonganti@101: flye_min_overlap = false kkonganti@101: flye_scaffold = false kkonganti@101: flye_meta = true kkonganti@101: ectyper_run = false kkonganti@101: ectyper_perc_opid = 90 kkonganti@101: ectyper_perc_hpid = 95 kkonganti@101: ectyper_perc_opcov = 95 kkonganti@101: ectyper_perc_hpcov = 50 kkonganti@101: serotypefinder_run = (params.centrifuge_extract_bug ==~ /(?i)Salmonella/ ? false : true) kkonganti@101: serotypefinder_db = '/tool-local-data/cfsan-centriflaken-db/0/serotypefinder/2.0.2' kkonganti@101: serotypefinder_min_cov = 0.80 kkonganti@101: serotypefinder_min_threshold = 0.85 kkonganti@101: serotypefinder_x = true kkonganti@101: seqsero2_run = (params.centrifuge_extract_bug ==~ /(?i)Salmonella/ ? true : false) kkonganti@101: seqsero2_t = 4 kkonganti@101: seqsero2_m = 'k' kkonganti@101: seqsero2_c = false kkonganti@101: seqsero2_s = false kkonganti@101: mlst_run = true kkonganti@101: mlst_minid = 95 kkonganti@101: mlst_mincov = 10 kkonganti@101: mlst_minscore = 50 kkonganti@101: amrfinderplus_run = false kkonganti@101: amrfinderplus_db = '/tool-local-data/cfsan-centriflaken-db/0/amrfinderplus/3.10.24/latest' kkonganti@101: amrfinderplus_genes = true kkonganti@101: abricate_run = true kkonganti@101: abricate_datadir = '/tool-local-data/cfsan-centriflaken-db/0/abricate/1.0.1/db' kkonganti@101: abricate_minid = 90 kkonganti@101: abricate_mincov = 80 kkonganti@101: abricate_summary_run = true kkonganti@101: seqkit_grep_on = false kkonganti@101: }