# HG changeset patch # User kkonganti # Date 1701114643 18000 # Node ID 6e5ceea338432e66005de0cf994114f53bacf6a8 # Parent 7ac696717239f96c542cb71351219a671e98fb31 "planemo upload" diff -r 7ac696717239 -r 6e5ceea33843 cfsan_cronology.xml --- a/cfsan_cronology.xml Mon Nov 27 14:37:10 2023 -0500 +++ b/cfsan_cronology.xml Mon Nov 27 14:50:43 2023 -0500 @@ -75,7 +75,7 @@ - @@ -115,11 +115,10 @@ **Purpose** -cronology is an automated workflow to assign Salmonella serotype based on NCBI Pathogen Detection Project for Salmonella. -It uses MASH to reduce the search space followed by additional genome filtering with sourmash. It then performs genome based -alignment with kma followed by count generation using salmon. This workflow can be used to analyze shotgun metagenomics -datasets, quasi-metagenomic datasets (enriched for Salmonella) and target enriched datasets (enriched with molecular baits specific for Salmonella) -and is especially useful in a case where a sample is of multi-serovar mixture. +cronology is an automated workflow for Cronobacter isolate assembly, +sequencing typing and traceback. The workflow version 0.1.0 takes in single-end +or paired-end Illumina short read data, performs QC using fastp, assembly and polish using shovill and polypolish +and whole genome distance based clustering using mashtree based on NCBI Pathogen Detection DB for Cronobacter. It is written in Nextflow and is part of the modular data analysis pipelines (CFSAN PIPELINES or CPIPES for short) at CFSAN. @@ -130,8 +129,7 @@ **Testing and Validation** -The CPIPES - cronology Nextflow pipeline has been wrapped to make it work in Galaxy. It takes in either paired or unpaired short reads list as an input -and performs read quality control followed by de novo assembly, gene prediction and annotation, sequence typing and whole genome distance based clustering. +The CPIPES - cronology Nextflow pipeline has been wrapped to make it work in Galaxy. All the testing has been done on the command line on the CFSAN Raven2 HPC Cluster.