Mercurial > repos > rliterman > csp2
comparison CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/opt/bbmap-39.01-1/fixgaps.sh @ 69:33d812a61356
planemo upload commit 2e9511a184a1ca667c7be0c6321a36dc4e3d116d
author | jpayne |
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date | Tue, 18 Mar 2025 17:55:14 -0400 |
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67:0e9998148a16 | 69:33d812a61356 |
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1 #!/bin/bash | |
2 | |
3 usage(){ | |
4 echo " | |
5 Written by Brian Bushnell | |
6 Last modified September 11, 2019 | |
7 | |
8 Description: Uses paired read insert sizes to estimate the correct | |
9 length of scaffold gaps, and resizes incorrectly-sized gaps. | |
10 | |
11 Usage: fixgaps.sh in=mapped.sam ref=scaffolds.fa out=fixed.fa | |
12 | |
13 Standard parameters: | |
14 in=<file> Reads mapped to the reference; should be sam or bam. | |
15 ref=<file> Reference; may be fasta or fastq. | |
16 out=<file> Modified reference; may be fasta or fastq. | |
17 overwrite=f (ow) Set to false to force the program to abort rather than | |
18 overwrite an existing file. | |
19 | |
20 Processing parameters: | |
21 gap=10 Consider any consecutive streak of Ns at least this long to | |
22 be a scaffold break. Gaps will not be resized to less than | |
23 this. | |
24 border=0.4 Ignore the outermost (border*readlen) of an insert (read pair) | |
25 when incrementing coverage. A higher value is more accurate | |
26 but requires more coverage and/or longer inserts. Range: 0-1. | |
27 mindepth=10 Minimum spanning read pairs to correct a gap. | |
28 | |
29 Java Parameters: | |
30 -Xmx This will set Java's memory usage, overriding autodetection. | |
31 -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will | |
32 specify 200 megs. The max is typically 85% of physical memory. | |
33 -eoom This flag will cause the process to exit if an out-of-memory | |
34 exception occurs. Requires Java 8u92+. | |
35 -da Disable assertions. | |
36 | |
37 Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. | |
38 " | |
39 } | |
40 | |
41 #This block allows symlinked shellscripts to correctly set classpath. | |
42 pushd . > /dev/null | |
43 DIR="${BASH_SOURCE[0]}" | |
44 while [ -h "$DIR" ]; do | |
45 cd "$(dirname "$DIR")" | |
46 DIR="$(readlink "$(basename "$DIR")")" | |
47 done | |
48 cd "$(dirname "$DIR")" | |
49 DIR="$(pwd)/" | |
50 popd > /dev/null | |
51 | |
52 #DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" | |
53 CP="$DIR""current/" | |
54 | |
55 z="-Xmx4g" | |
56 z2="-Xms4g" | |
57 set=0 | |
58 | |
59 if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then | |
60 usage | |
61 exit | |
62 fi | |
63 | |
64 calcXmx () { | |
65 source "$DIR""/calcmem.sh" | |
66 setEnvironment | |
67 parseXmx "$@" | |
68 if [[ $set == 1 ]]; then | |
69 return | |
70 fi | |
71 freeRam 4000m 84 | |
72 z="-Xmx${RAM}m" | |
73 z2="-Xms${RAM}m" | |
74 } | |
75 calcXmx "$@" | |
76 | |
77 fixgaps() { | |
78 local CMD="java $EA $EOOM $z -cp $CP consensus.FixScaffoldGaps $@" | |
79 echo $CMD >&2 | |
80 eval $CMD | |
81 } | |
82 | |
83 fixgaps "$@" |