Mercurial > repos > rliterman > csp2
comparison CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/opt/bbmap-39.01-1/kmerlimit.sh @ 69:33d812a61356
planemo upload commit 2e9511a184a1ca667c7be0c6321a36dc4e3d116d
author | jpayne |
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date | Tue, 18 Mar 2025 17:55:14 -0400 |
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67:0e9998148a16 | 69:33d812a61356 |
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1 #!/bin/bash | |
2 | |
3 usage(){ | |
4 echo " | |
5 Written by Brian Bushnell | |
6 Last modified July 31, 2018 | |
7 | |
8 Description: Stops producing reads when the unique kmer limit is reached. | |
9 This is approximate. If the input has been Clumpified, the order should be | |
10 randomized first with shuffle2.sh or sortbyname.sh with the flowcell flag. | |
11 | |
12 Differences between versions: | |
13 kmerlimit.sh uses 1 pass and outputs all reads until a limit is hit, | |
14 meaning the input reads should be in random order with respect to sequence. | |
15 kmerlimit2.sh uses 2 passes and randomly subsamples from the file, so | |
16 it works with reads in any order. | |
17 | |
18 Usage: kmerlimit.sh in=<input file> out=<output file> limit=<number> | |
19 | |
20 Standard parameters: | |
21 in=<file> Primary input, or read 1 input. | |
22 in2=<file> Read 2 input if reads are in two files. | |
23 out=<file> Primary output, or read 1 output. | |
24 out2=<file> Read 2 output if reads are in two files. | |
25 overwrite=t (ow) Set to false to force the program to abort rather than | |
26 overwrite an existing file. | |
27 ziplevel=2 (zl) Set to 1 (lowest) through 9 (max) to change compression | |
28 level; lower compression is faster. | |
29 | |
30 Processing parameters: | |
31 k=31 Kmer length, 1-32. | |
32 limit= The number of unique kmers to produce. | |
33 mincount=1 Ignore kmers seen fewer than this many times. | |
34 minqual=0 Ignore bases with quality below this. | |
35 minprob=0.2 Ignore kmers with correctness probability below this. | |
36 | |
37 Java Parameters: | |
38 -Xmx This will set Java's memory usage, overriding autodetection. | |
39 -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will | |
40 specify 200 megs. The max is typically 85% of physical memory. | |
41 -eoom This flag will cause the process to exit if an out-of-memory | |
42 exception occurs. Requires Java 8u92+. | |
43 -da Disable assertions. | |
44 | |
45 Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. | |
46 " | |
47 } | |
48 | |
49 #This block allows symlinked shellscripts to correctly set classpath. | |
50 pushd . > /dev/null | |
51 DIR="${BASH_SOURCE[0]}" | |
52 while [ -h "$DIR" ]; do | |
53 cd "$(dirname "$DIR")" | |
54 DIR="$(readlink "$(basename "$DIR")")" | |
55 done | |
56 cd "$(dirname "$DIR")" | |
57 DIR="$(pwd)/" | |
58 popd > /dev/null | |
59 | |
60 #DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" | |
61 CP="$DIR""current/" | |
62 | |
63 z="-Xmx1000m" | |
64 z2="-Xms1000m" | |
65 set=0 | |
66 | |
67 if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then | |
68 usage | |
69 exit | |
70 fi | |
71 | |
72 calcXmx () { | |
73 source "$DIR""/calcmem.sh" | |
74 setEnvironment | |
75 parseXmx "$@" | |
76 } | |
77 calcXmx "$@" | |
78 | |
79 kmerlimit() { | |
80 local CMD="java $EA $EOOM $z -cp $CP sketch.KmerLimit $@" | |
81 echo $CMD >&2 | |
82 eval $CMD | |
83 } | |
84 | |
85 kmerlimit "$@" |