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1 '\" t
2 .\" Title: mash-screen
3 .\" Author: [see the "AUTHOR(S)" section]
4 .\" Generator: Asciidoctor 2.0.10
5 .\" Date: 2019-12-13
6 .\" Manual: \ \&
7 .\" Source: \ \&
8 .\" Language: English
9 .\"
10 .TH "MASH\-SCREEN" "1" "2019-12-13" "\ \&" "\ \&"
11 .ie \n(.g .ds Aq \(aq
12 .el .ds Aq '
13 .ss \n[.ss] 0
14 .nh
15 .ad l
16 .de URL
17 \fI\\$2\fP <\\$1>\\$3
18 ..
19 .als MTO URL
20 .if \n[.g] \{\
21 . mso www.tmac
22 . am URL
23 . ad l
24 . .
25 . am MTO
26 . ad l
27 . .
28 . LINKSTYLE blue R < >
29 .\}
30 .SH "NAME"
31 mash\-screen \- determine whether query sequences are within a larger pool of sequences
32 .SH "SYNOPSIS"
33 .sp
34 \fBmash screen\fP [options] <queries>.msh <pool> [<pool>] ...
35 .SH "DESCRIPTION"
36 .sp
37 Determine how well query sequences are contained within a pool of sequences.
38 The queries must be formatted as a single Mash sketch file (.msh), created
39 with the \f(CRmash sketch\fP command. The <pool> files can be contigs or reads, in
40 fasta or fastq, gzipped or not, and "\-" can be given for <pool> to read from
41 standard input. The <pool> sequences are assumed to be nucleotides, and will
42 be 6\-frame translated if the <queries> are amino acids. The output fields are
43 [identity, shared\-hashes, median\-multiplicity, p\-value, query\-ID, query\-comment],
44 where median\-multiplicity is computed for shared hashes, based on the number of
45 observations of those hashes within the pool.
46 .SH "OPTIONS"
47 .sp
48 \fB\-h\fP
49 .RS 4
50 Help
51 .RE
52 .sp
53 \fB\-p\fP <int>
54 .RS 4
55 Parallelism. This many threads will be spawned for processing.
56 .RE
57 .sp
58 \fB\-w\fP
59 .RS 4
60 Winner\-takes\-all strategy for identity estimates. After counting
61 hashes for each query, hashes that appear in multiple queries will
62 be removed from all except the one with the best identity (ties
63 broken by larger query), and other identities will be reduced. This
64 removes output redundancy, providing a rough compositional outline.
65 .RE
66 .SS "Output"
67 .sp
68 \fB\-i\fP <num>
69 .RS 4
70 Minimum identity to report. Inclusive unless set to zero, in which
71 case only identities greater than zero (i.e. with at least one
72 shared hash) will be reported. Set to \-1 to output everything.
73 .RE
74 .sp
75 \fB\-v\fP <num>
76 .RS 4
77 Maximum p\-value to report.
78 .RE
79 .SH "SEE ALSO"
80 .sp
81 mash(1)