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comparison CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/share/man/man1/mash-screen.1 @ 68:5028fdace37b
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author | jpayne |
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date | Tue, 18 Mar 2025 16:23:26 -0400 |
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1 '\" t | |
2 .\" Title: mash-screen | |
3 .\" Author: [see the "AUTHOR(S)" section] | |
4 .\" Generator: Asciidoctor 2.0.10 | |
5 .\" Date: 2019-12-13 | |
6 .\" Manual: \ \& | |
7 .\" Source: \ \& | |
8 .\" Language: English | |
9 .\" | |
10 .TH "MASH\-SCREEN" "1" "2019-12-13" "\ \&" "\ \&" | |
11 .ie \n(.g .ds Aq \(aq | |
12 .el .ds Aq ' | |
13 .ss \n[.ss] 0 | |
14 .nh | |
15 .ad l | |
16 .de URL | |
17 \fI\\$2\fP <\\$1>\\$3 | |
18 .. | |
19 .als MTO URL | |
20 .if \n[.g] \{\ | |
21 . mso www.tmac | |
22 . am URL | |
23 . ad l | |
24 . . | |
25 . am MTO | |
26 . ad l | |
27 . . | |
28 . LINKSTYLE blue R < > | |
29 .\} | |
30 .SH "NAME" | |
31 mash\-screen \- determine whether query sequences are within a larger pool of sequences | |
32 .SH "SYNOPSIS" | |
33 .sp | |
34 \fBmash screen\fP [options] <queries>.msh <pool> [<pool>] ... | |
35 .SH "DESCRIPTION" | |
36 .sp | |
37 Determine how well query sequences are contained within a pool of sequences. | |
38 The queries must be formatted as a single Mash sketch file (.msh), created | |
39 with the \f(CRmash sketch\fP command. The <pool> files can be contigs or reads, in | |
40 fasta or fastq, gzipped or not, and "\-" can be given for <pool> to read from | |
41 standard input. The <pool> sequences are assumed to be nucleotides, and will | |
42 be 6\-frame translated if the <queries> are amino acids. The output fields are | |
43 [identity, shared\-hashes, median\-multiplicity, p\-value, query\-ID, query\-comment], | |
44 where median\-multiplicity is computed for shared hashes, based on the number of | |
45 observations of those hashes within the pool. | |
46 .SH "OPTIONS" | |
47 .sp | |
48 \fB\-h\fP | |
49 .RS 4 | |
50 Help | |
51 .RE | |
52 .sp | |
53 \fB\-p\fP <int> | |
54 .RS 4 | |
55 Parallelism. This many threads will be spawned for processing. | |
56 .RE | |
57 .sp | |
58 \fB\-w\fP | |
59 .RS 4 | |
60 Winner\-takes\-all strategy for identity estimates. After counting | |
61 hashes for each query, hashes that appear in multiple queries will | |
62 be removed from all except the one with the best identity (ties | |
63 broken by larger query), and other identities will be reduced. This | |
64 removes output redundancy, providing a rough compositional outline. | |
65 .RE | |
66 .SS "Output" | |
67 .sp | |
68 \fB\-i\fP <num> | |
69 .RS 4 | |
70 Minimum identity to report. Inclusive unless set to zero, in which | |
71 case only identities greater than zero (i.e. with at least one | |
72 shared hash) will be reported. Set to \-1 to output everything. | |
73 .RE | |
74 .sp | |
75 \fB\-v\fP <num> | |
76 .RS 4 | |
77 Maximum p\-value to report. | |
78 .RE | |
79 .SH "SEE ALSO" | |
80 .sp | |
81 mash(1) |