Mercurial > repos > rliterman > csp2
diff CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/opt/bbmap-39.01-1/addadapters.sh @ 69:33d812a61356
planemo upload commit 2e9511a184a1ca667c7be0c6321a36dc4e3d116d
author | jpayne |
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date | Tue, 18 Mar 2025 17:55:14 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/opt/bbmap-39.01-1/addadapters.sh Tue Mar 18 17:55:14 2025 -0400 @@ -0,0 +1,78 @@ +#!/bin/bash + +usage(){ +echo " +Written by Brian Bushnell +Last modified February 17, 2015 + +***DEPRECATED*** + +Description: Randomly adds adapters to a file, or grades a trimmed file. +The input is a set of reads, paired or unpaired. +The output is those same reads with adapter sequence replacing some of the bases in some reads. +For paired reads, adapters are located in the same position in read1 and read2. +This is designed for benchmarking adapter-trimming software, and evaluating methodology. +randomreads.sh is better for paired reads, though, as it actually adds adapters at the correct location, +so that overlap may be used for adapter detection. + +Usage: addadapters.sh in=<file> in2=<file2> out=<outfile> out2=<outfile2> adapters=<file> + +in2 and out2 are for paired reads and are optional. +If input is paired and there is only one output file, it will be written interleaved. + +Parameters: +ow=f (overwrite) Overwrites files that already exist. +int=f (interleaved) Determines whether INPUT file is considered interleaved. +qin=auto ASCII offset for input quality. May be 33 (Sanger), 64 (Illumina), or auto. +qout=auto ASCII offset for output quality. May be 33 (Sanger), 64 (Illumina), or auto (same as input). +add Add adapters to input files. Default mode. +grade Evaluate trimmed input files. +adapters=<file> Fasta file of adapter sequences. +literal=<sequence> Comma-delimited list of adapter sequences. +left Adapters are on the left (3') end of the read. +right Adapters are on the right (5') end of the read. Default mode. +adderrors=t Add errors to adapters based on the quality scores. +addpaired=t Add adapters to the same location for read 1 and read 2. +arc=f Add reverse-complemented adapters as well as forward. +rate=0.5 Add adapters to this fraction of reads. + +Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. +" +} + +#This block allows symlinked shellscripts to correctly set classpath. +pushd . > /dev/null +DIR="${BASH_SOURCE[0]}" +while [ -h "$DIR" ]; do + cd "$(dirname "$DIR")" + DIR="$(readlink "$(basename "$DIR")")" +done +cd "$(dirname "$DIR")" +DIR="$(pwd)/" +popd > /dev/null + +#DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" +CP="$DIR""current/" + +z="-Xmx200m" +set=0 + +if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then + usage + exit +fi + +calcXmx () { + source "$DIR""/calcmem.sh" + setEnvironment + parseXmx "$@" +} +calcXmx "$@" + +function addadapters() { + local CMD="java $EA $EOOM $z -cp $CP jgi.AddAdapters $@" + echo $CMD >&2 + eval $CMD +} + +addadapters "$@"