Mercurial > repos > rliterman > csp2
diff CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/opt/bbmap-39.01-1/bbcountunique.sh @ 69:33d812a61356
planemo upload commit 2e9511a184a1ca667c7be0c6321a36dc4e3d116d
author | jpayne |
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date | Tue, 18 Mar 2025 17:55:14 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/opt/bbmap-39.01-1/bbcountunique.sh Tue Mar 18 17:55:14 2025 -0400 @@ -0,0 +1,93 @@ +#!/bin/bash + +usage(){ +echo " +Written by Brian Bushnell +Last modified August 1, 2016 + +Description: Generates a kmer uniqueness histogram, binned by file position. +There are 3 columns for single reads, 6 columns for paired: +count number of reads or pairs processed +r1_first percent unique 1st kmer of read 1 +r1_rand percent unique random kmer of read 1 +r2_first percent unique 1st kmer of read 2 +r2_rand percent unique random kmer of read 2 +pair percent unique concatenated kmer from read 1 and 2 + +Please read bbmap/docs/guides/CalcUniquenessGuide.txt for more information. + +Usage: bbcountunique.sh in=<input> out=<output> + +Input parameters: +in2=null Second input file for paired reads +interleaved=auto Set true/false to override autodetection of the input file as paired interleaved. +samplerate=1 Set to below 1 to sample a fraction of input reads. +reads=-1 Only process this number of reads, then quit (-1 means all) + +Output parameters: +out=<file> File for output stats + +Processing parameters: +k=25 Kmer length (range 1-31). +interval=25000 Print one line to the histogram per this many reads. +cumulative=f Show cumulative numbers rather than per-interval numbers. +percent=t Show percentages of unique reads. +count=f Show raw counts of unique reads. +printlastbin=f (plb) Print a line for the final undersized bin. +minprob=0 Ignore kmers with a probability of correctness below this (based on q-scores). + +Java Parameters: +-Xmx This will set Java's memory usage, overriding autodetection. + -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will specify 200 megs. + The max is typically 85% of physical memory. +-eoom This flag will cause the process to exit if an + out-of-memory exception occurs. Requires Java 8u92+. +-da Disable assertions. + +Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. +" +} + +#This block allows symlinked shellscripts to correctly set classpath. +pushd . > /dev/null +DIR="${BASH_SOURCE[0]}" +while [ -h "$DIR" ]; do + cd "$(dirname "$DIR")" + DIR="$(readlink "$(basename "$DIR")")" +done +cd "$(dirname "$DIR")" +DIR="$(pwd)/" +popd > /dev/null + +#DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" +CP="$DIR""current/" + +z="-Xmx1g" +z2="-Xms1g" +set=0 + +if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then + usage + exit +fi + +calcXmx () { + source "$DIR""/calcmem.sh" + setEnvironment + parseXmx "$@" + if [[ $set == 1 ]]; then + return + fi + freeRam 3200m 84 + z="-Xmx${RAM}m" + z2="-Xms${RAM}m" +} +calcXmx "$@" + +bbcountunique() { + local CMD="java $EA $EOOM $z $z2 -cp $CP jgi.CalcUniqueness $@" + echo $CMD >&2 + eval $CMD +} + +bbcountunique "$@"