Mercurial > repos > rliterman > csp2
diff CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/opt/bbmap-39.01-1/bbsplitpairs.sh @ 69:33d812a61356
planemo upload commit 2e9511a184a1ca667c7be0c6321a36dc4e3d116d
author | jpayne |
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date | Tue, 18 Mar 2025 17:55:14 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/opt/bbmap-39.01-1/bbsplitpairs.sh Tue Mar 18 17:55:14 2025 -0400 @@ -0,0 +1,82 @@ +#!/bin/bash + +usage(){ +echo " +Written by Brian Bushnell +Last modified February 17, 2015 + +Description: Separates paired reads into files of 'good' pairs and 'good' singletons by removing 'bad' reads that are shorter than a min length. +Designed to handle situations where reads become too short to be useful after trimming. This program also optionally performs quality trimming. + +Usage: bbsplitpairs.sh in=<input file> out=<pair output file> outs=<singleton output file> minlen=<minimum read length, an integer> + +Input may be fasta or fastq, compressed or uncompressed. + +Optional parameters (and their defaults) + +in=<file> The 'in=' flag is needed if the input file is not the first parameter. 'in=stdin' will pipe from standard in. +in2=<file> Use this if 2nd read of pairs are in a different file. +out=<file> The 'out=' flag is needed if the output file is not the second parameter. 'out=stdout' will pipe to standard out. +out2=<file> Use this to write 2nd read of pairs to a different file. +outsingle=<file> (outs) Write singleton reads here. + +overwrite=t (ow) Set to false to force the program to abort rather than overwrite an existing file. +showspeed=t (ss) Set to 'f' to suppress display of processing speed. +interleaved=auto (int) If true, forces fastq input to be paired and interleaved. +qtrim=f Trim read ends to remove bases with quality below trimq. + Values: rl (trim both ends), f (neither end), r (right end only), l (left end only). +trimq=6 Trim quality threshold. +minlen=20 (ml) Reads shorter than this after trimming will be discarded. +ziplevel=2 (zl) Set to 1 (lowest) through 9 (max) to change compression level; lower compression is faster. +fixinterleaving=f (fint) Fixes corrupted interleaved files by examining pair names. Only use on files with broken interleaving. +repair=f (rp) Fixes arbitrarily corrupted paired reads by examining read names. High memory. +ain=f (allowidenticalnames) When detecting pair names, allows identical names, instead of requiring /1 and /2 or 1: and 2: + +Java Parameters: +-Xmx This will set Java's memory usage, overriding autodetection. + -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will specify 200 megs. + The max is typically 85% of physical memory. +-eoom This flag will cause the process to exit if an + out-of-memory exception occurs. Requires Java 8u92+. +-da Disable assertions. + +Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. +" +} + +#This block allows symlinked shellscripts to correctly set classpath. +pushd . > /dev/null +DIR="${BASH_SOURCE[0]}" +while [ -h "$DIR" ]; do + cd "$(dirname "$DIR")" + DIR="$(readlink "$(basename "$DIR")")" +done +cd "$(dirname "$DIR")" +DIR="$(pwd)/" +popd > /dev/null + +#DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" +CP="$DIR""current/" + +z="-Xmx200m" +set=0 + +if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then + usage + exit +fi + +calcXmx () { + source "$DIR""/calcmem.sh" + setEnvironment + parseXmx "$@" +} +calcXmx "$@" + +splitpairs() { + local CMD="java $EA $EOOM $z -cp $CP jgi.SplitPairsAndSingles $@" + echo $CMD >&2 + eval $CMD +} + +splitpairs "$@"