Mercurial > repos > rliterman > csp2
diff CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/opt/bbmap-39.01-1/kmercountmulti.sh @ 69:33d812a61356
planemo upload commit 2e9511a184a1ca667c7be0c6321a36dc4e3d116d
author | jpayne |
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date | Tue, 18 Mar 2025 17:55:14 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/opt/bbmap-39.01-1/kmercountmulti.sh Tue Mar 18 17:55:14 2025 -0400 @@ -0,0 +1,84 @@ +#!/bin/bash + +usage(){ +echo " +Written by Brian Bushnell +Last modified February 20, 2020 + +Description: Estimates cardinality of unique kmers in sequence data. +Processes multiple kmer lengths simultaneously to produce a histogram. + +Usage: kmercountmulti.sh in=<file> sweep=<20,100,8> out=<histogram output> + +Parameters: +in=<file> (in1) Input file, or comma-delimited list of files. +in2=<file> Optional second file for paired reads. +out=<file> Histogram output. Default is stdout. +k= Comma-delimited list of kmer lengths to use. +sweep=min,max,incr Use incremented kmer values from min to max. For example, + sweep=20,26,2 is equivalent to k=20,22,24,26. +buckets=2048 Use this many buckets for counting; higher decreases + variance, for large datasets. Must be a power of 2. +seed=-1 Use this seed for hash functions. + A negative number forces a random seed. +minprob=0 Set to a value between 0 and 1 to exclude kmers with a + lower probability of being correct. +hashes=1 Use this many hash functions. More hashes yield greater + accuracy, but H hashes takes H times as long. +stdev=f Print standard deviations. + +Shortcuts: +The # symbol will be substituted for 1 and 2. +For example: +kmercountmulti.sh in=read#.fq +...is equivalent to: +kmercountmulti.sh in1=read1.fq in2=read2.fq + +Java Parameters: +-Xmx This will set Java's memory usage, overriding autodetection. + -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will specify 200 megs. + The max is typically 85% of physical memory. +-eoom This flag will cause the process to exit if an + out-of-memory exception occurs. Requires Java 8u92+. +-da Disable assertions. + +Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. +" +} + +#This block allows symlinked shellscripts to correctly set classpath. +pushd . > /dev/null +DIR="${BASH_SOURCE[0]}" +while [ -h "$DIR" ]; do + cd "$(dirname "$DIR")" + DIR="$(readlink "$(basename "$DIR")")" +done +cd "$(dirname "$DIR")" +DIR="$(pwd)/" +popd > /dev/null + +#DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" +CP="$DIR""current/" + +z="-Xmx500m" +set=0 + +if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then + usage + exit +fi + +calcXmx () { + source "$DIR""/calcmem.sh" + setEnvironment + parseXmx "$@" +} +calcXmx "$@" + +function kmercountmulti() { + local CMD="java $EA $EOOM $z -cp $CP jgi.KmerCountMulti $@" + echo $CMD >&2 + eval $CMD +} + +kmercountmulti "$@"