--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/csp2-screen.xml	Tue Mar 18 17:55:14 2025 -0400
@@ -0,0 +1,158 @@
+<tool id="csp2-screen" name="CSP2 (Screening Mode)" version="0.9.7_Dev21">
+        <description>Screen query assemblies against reference assemblies</description>
+        <requirements>
+                <!-- <requirement type="package" version="24.10.1">nextflow</requirement>
+                <requirement type="package" version="1.5.8">micromamba</requirement> -->
+                <container type="docker">cfsanbiostatistics/csp2:v.0.9.7.4</container>
+        </requirements>
+        <version_command>nextflow -version</version_command>
+        <command detect_errors="exit_code"><![CDATA[
+mkdir -p queries references
+#set readext=""
+#for $reads in $query.coll
+#if $hasattr($reads, "is_of_type") and $reads.is_of_type("fasta")
+         && ln -sf ${reads} 'queries/${reads.element_identifier}.fasta'
+#else if $reads.forward.is_of_type("fastq.gz","fastqsanger.gz")
+         #set readext="fastq.gz"
+         && ln -sf '${reads.forward}' 'queries/${reads.forward.element_identifier}_1.fastq.gz'
+         && ln -sf '${reads.reverse}' 'queries/${reads.reverse.element_identifier}_2.fastq.gz'
+#else if $reads.forward.is_of_type("fastq.bz2", "fastqsanger.bz2")
+         #set readext="fastq.bz2"
+         && ln -sf '${reads.forward}' 'queries/${reads.forward.element_identifier}_1.fastq.bz2'
+         && ln -sf '${reads.reverse}' 'queries/${reads.reverse.element_identifier}_2.fastq.bz2'
+#else 
+         #set readext="fastq"
+         && ln -sf '${reads.forward}' 'queries/${reads.forward.element_identifier}_1.fastq'
+         && ln -sf '${reads.reverse}' 'queries/${reads.reverse.element_identifier}_2.fastq'
+#end if
+#end for
+        && echo "*** Files in queries directory: ***"
+        && ls -lah queries/
+        && ln -sf '$source.reference' 'references/${source.reference.element_identifier}.fasta'
+        && nextflow run 
+        ${__tool_directory__}/CSP2/CSP2.nf -profile csp2_galaxy
+        --runmode screen 
+        #if $query.query_select == 'reads'
+        --reads queries
+        --readext $readext 
+        --forward "_1.${readext}"
+        --reverse "_2.${readext}"
+        #else
+        --fasta queries
+        #end if
+        --ref_fasta 'references/${source.reference.element_identifier}.fasta'
+        --min_cov $opt.min_cov 
+        --min_iden $opt.min_iden 
+        --min_len $opt.min_len 
+        --ref_edge $opt.ref_edge 
+        --query_edge $opt.query_edge 
+        --dwin $opt.dwin 
+        --wsnps $opt.wsnps 
+        --out CSP2_Screen_Output
+        && echo "*** Files in output directory: ***"
+        && ls -lah CSP2_Screen_Output
+        && echo "*** Nextflow log follows: ***"
+        && cat .nextflow.log
+]]>
+        </command>
+        <inputs>
+                <conditional name="source">
+                        <param name="source_select" type="select" label="Use a curated GalaxyTrakr reference or a reference from your history">
+                                <option value="curated">Use a GalaxyTrakr reference</option>
+                                <option value="history">Use a reference from your history</option>
+                        </param>
+                        <when value="curated">
+                                <param name="reference" type="select" label="Select reference fasta">
+                                        <options from_data_table="all_fasta">
+                                                <filter type="sort_by" column="2"/>
+                                                <validator type="no_options" message="No assemblies are available for the selected input dataset"/>
+                                        </options>
+                                </param>
+                        </when>
+                        <when value="history">
+                                <param type="data" name="reference" format="fasta" label="Select reference FASTA"/>
+                        </when>
+                </conditional>
+                <conditional name="query">
+                        <param name="query_select" type="select" label="Screen a list of paired-end reads or a list of assemblies">
+                                <option value="reads">Screen a list of paired reads</option>
+                                <option value="assemblies">Screen a list of assemblies</option>
+                        </param>
+                        <when value="reads">
+                                <param label="Paired reads" name="coll" type="data_collection" format="fastq,fastqsanger,fastq.gz,fastqsanger.gz,fastq.bz2,fastqsanger.bz2" collection_type="list:paired" />
+                        </when>
+                        <when value="assemblies">
+                                <param label="Assemblies" name="coll" type="data_collection" format="fasta" collection_type="list" />
+                        </when>
+                </conditional>
+                <section name="opt" title="Advanced options...">
+                        <param argument="--min_cov" name="min_cov" type="float" value="85" label="Minimum reference genome coverage to proceed with distance estimation" />
+                        <param argument="--min_eden" name="min_iden" type="float" value="99" label="Minimum alignment percent identity to detect SNPs" />
+                        <param argument="--min_len" name="min_len" type="integer" value="500" label="Minimum alignment length to detect SNPs" />
+                        <param argument="--ref_edge" name="ref_edge" type="integer" value="150" label="Prune SNPs within this many bases of reference contig edge" />
+                        <param argument="--query_edge" name="query_edge" type="integer" value="150" label="Prune SNPs within this many bases of query contig edge" />
+                        <param argument="--dwin" name="dwin" type="text" value="1000,125,15" label="Comma-separated set of window sizes for SNP density filtration (Set to 0 to disable density filtration)" />
+                        <param argument="--wsnips" name="wsnps" type="text" value="3,2,1" label="Comma-separated list of maximum SNP counts per density window" />
+                </section>
+        </inputs>
+        <outputs>
+                <data name="raw_mummer" format="tabular" label="Raw MUMmer Output" from_work_dir="CSP2_Screen_Output/Raw_MUMmer_Summary.tsv" />
+                <data name="isolate_data" format="tabular" label="Isolate Data" from_work_dir="CSP2_Screen_Output/Isolate_Data.tsv" />
+                <data name="screening_results" format="tabular" label="Screening Results" from_work_dir="CSP2_Screen_Output/Screening_Results.tsv" />
+                <!-- <data name="nextflow_log" format="txt" label="Nextflow Log" from_work_dir="Nextflow_Log.txt" /> -->
+        </outputs>
+        <tests>
+                <test>
+                        <param name="source_select" value="history" />
+                        <param name="reference" value="assemblies/Sample_A.fasta" ftype="fasta" />
+                        <param name="query_select" value="assemblies" />
+                        <param name="coll">
+                                <collection type="list">
+                                        <!-- <element name="Sample_A" value="assemblies/Sample_A.fasta" /> -->
+                                        <element name="Sample_B" value="assemblies/Sample_B.fasta" />
+                                        <element name="Sample_C" value="assemblies/Sample_C.fasta" />
+                                        <element name="Sample_D" value="assemblies/Sample_D.fasta" />
+                                        <element name="Sample_E" value="assemblies/Sample_E.fasta" />
+                                        <element name="Sample_F" value="assemblies/Sample_F.fasta" />
+                                        <element name="Sample_G" value="assemblies/Sample_G.fasta" />
+                                        <element name="Sample_H" value="assemblies/Sample_H.fasta" />
+                                        <element name="Sample_I" value="assemblies/Sample_I.fasta" />
+                                        <element name="Sample_J" value="assemblies/Sample_J.fasta" />
+                                        <element name="Sample_K" value="assemblies/Sample_K.fasta" />
+                                        <element name="Sample_L" value="assemblies/Sample_L.fasta" />
+                                        <element name="Sample_M" value="assemblies/Sample_M.fasta" />
+                                        <element name="Sample_N" value="assemblies/Sample_N.fasta" />
+                                        <element name="Sample_O" value="assemblies/Sample_O.fasta" />
+                                </collection>
+                        </param>
+
+                        <output name="screening_results" value="Screening_Results.tsv" />
+                        <output name="isolate_data" value="Isolate_Data.tsv" />
+                </test>
+                <test>
+                        <param name="source_select" value="history" />
+                        <param name="reference" value="assemblies/Sample_A.fasta" ftype="fasta" />
+                        <param name="query_select" value="reads" />
+                        <param name="coll">
+                                <collection type="list:paired">
+                                        <element name="Sample_A" >
+                                                <collection type="paired">
+                                                        <element name="forward" value="reads/Week_42_Reads_1.fq.gz" ftype="fastqsanger.gz" />
+                                                        <element name="reverse" value="reads/Week_42_Reads_2.fq.gz" ftype="fastqsanger.gz" />
+                                                </collection>
+                                        </element>
+                                </collection>
+                        </param>
+                        <output name="screening_results" value="Screening_Results.tsv" />
+                        <output name="isolate_data" value="Isolate_Data.tsv" />
+                </test>
+        </tests>
+        <help>
+        This tool takes query assemblies and reference assemblies and calculates the pairwise distance between each query/reference combination. If no reference is provided, all queries are compared to all other queries.
+        </help>
+        <citations>
+                <citation type="doi">10.XXXX/placeholder.doi</citation>
+                <citation type="bibtex">@article{example2024,title={CFSAN SNP Pipeline 2 (CSP2): a pipeline for fast and accurate SNP distance estimation from bacterial genome assemblies.},author={Doe, John and Smith, Jane},journal={Submitted},year={2024}}
+                </citation>
+        </citations>
+</tool>