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author | jpayne |
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date | Tue, 18 Mar 2025 17:55:14 -0400 |
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#!/bin/bash usage(){ echo " Written by Brian Bushnell Last modified November 9, 2016 Description: Re-pairs reads that became disordered or had some mates eliminated. Please read bbmap/docs/guides/RepairGuide.txt for more information. Usage: repair.sh in=<input file> out=<pair output> outs=<singleton output> Input may be fasta, fastq, or sam, compressed or uncompressed. Parameters: in=<file> The 'in=' flag is needed if the input file is not the first parameter. 'in=stdin' will pipe from standard in. in2=<file> Use this if 2nd read of pairs are in a different file. out=<file> The 'out=' flag is needed if the output file is not the second parameter. 'out=stdout' will pipe to standard out. out2=<file> Use this to write 2nd read of pairs to a different file. outs=<file> (outsingle) Write singleton reads here. overwrite=t (ow) Set to false to force the program to abort rather than overwrite an existing file. showspeed=t (ss) Set to 'f' to suppress display of processing speed. ziplevel=2 (zl) Set to 1 (lowest) through 9 (max) to change compression level; lower compression is faster. fint=f (fixinterleaving) Fixes corrupted interleaved files using read names. Only use on files with broken interleaving - correctly interleaved files from which some reads were removed. repair=t (rp) Fixes arbitrarily corrupted paired reads by using read names. Uses much more memory than 'fint' mode. ain=f (allowidenticalnames) When detecting pair names, allows identical names, instead of requiring /1 and /2 or 1: and 2: Java Parameters: -Xmx This will set Java's memory usage, overriding autodetection. -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will specify 200 megs. The max is typically 85% of physical memory. -eoom This flag will cause the process to exit if an out-of-memory exception occurs. Requires Java 8u92+. -da Disable assertions. Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. " } #This block allows symlinked shellscripts to correctly set classpath. pushd . > /dev/null DIR="${BASH_SOURCE[0]}" while [ -h "$DIR" ]; do cd "$(dirname "$DIR")" DIR="$(readlink "$(basename "$DIR")")" done cd "$(dirname "$DIR")" DIR="$(pwd)/" popd > /dev/null #DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" CP="$DIR""current/" z="-Xmx4g" z2="-Xms4g" set=0 if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then usage exit fi calcXmx () { source "$DIR""/calcmem.sh" setEnvironment parseXmx "$@" if [[ $set == 1 ]]; then return fi freeRam 4000m 84 z="-Xmx${RAM}m" z2="-Xms${RAM}m" } calcXmx "$@" repair() { local CMD="java $EA $EOOM $z -cp $CP jgi.SplitPairsAndSingles rp $@" echo $CMD >&2 eval $CMD } repair "$@"