jpayne@69: #!/bin/bash jpayne@69: jpayne@69: usage(){ jpayne@69: echo " jpayne@69: Written by Brian Bushnell jpayne@69: Last modified February 20, 2015 jpayne@69: jpayne@69: Description: Prints the most common kmers in each sequence. jpayne@69: This is intended for short kmers only! jpayne@69: jpayne@69: Usage: commonkmers.sh in= out= jpayne@69: jpayne@69: Parameters: jpayne@69: k=2 Kmer length, 0-12. jpayne@69: display=3 Print this many kmers per sequence. jpayne@69: count=f Print the kmer counts as well. jpayne@69: jpayne@69: ow=f (overwrite) Overwrites files that already exist. jpayne@69: app=f (append) Append to files that already exist. jpayne@69: zl=4 (ziplevel) Set compression level, 1 (low) to 9 (max). jpayne@69: qin=auto ASCII offset for input quality. May be 33 (Sanger), 64 (Illumina), or auto. jpayne@69: jpayne@69: Java Parameters: jpayne@69: -Xmx This will set Java's memory usage, overriding autodetection. jpayne@69: -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will specify 200 megs. jpayne@69: The max is typically 85% of physical memory. jpayne@69: -eoom This flag will cause the process to exit if an out-of-memory jpayne@69: exception occurs. Requires Java 8u92+. jpayne@69: -da Disable assertions. jpayne@69: jpayne@69: Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. jpayne@69: " jpayne@69: } jpayne@69: jpayne@69: #This block allows symlinked shellscripts to correctly set classpath. jpayne@69: pushd . > /dev/null jpayne@69: DIR="${BASH_SOURCE[0]}" jpayne@69: while [ -h "$DIR" ]; do jpayne@69: cd "$(dirname "$DIR")" jpayne@69: DIR="$(readlink "$(basename "$DIR")")" jpayne@69: done jpayne@69: cd "$(dirname "$DIR")" jpayne@69: DIR="$(pwd)/" jpayne@69: popd > /dev/null jpayne@69: jpayne@69: #DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" jpayne@69: CP="$DIR""current/" jpayne@69: jpayne@69: z="-Xmx800m" jpayne@69: set=0 jpayne@69: jpayne@69: if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then jpayne@69: usage jpayne@69: exit jpayne@69: fi jpayne@69: jpayne@69: calcXmx () { jpayne@69: source "$DIR""/calcmem.sh" jpayne@69: setEnvironment jpayne@69: parseXmx "$@" jpayne@69: } jpayne@69: calcXmx "$@" jpayne@69: jpayne@69: function commonkmers() { jpayne@69: local CMD="java $EA $EOOM $z -cp $CP jgi.SmallKmerFrequency $@" jpayne@69: echo $CMD >&2 jpayne@69: eval $CMD jpayne@69: } jpayne@69: jpayne@69: commonkmers "$@"