jpayne@69: #!/bin/bash
jpayne@69:
jpayne@69: usage(){
jpayne@69: echo "
jpayne@69: Written by Brian Bushnell
jpayne@69: Last modified December 4, 2019
jpayne@69:
jpayne@69: Description: Aligns SSUs to each other and reports identity.
jpayne@69: This requires sequences annotated with a taxID in their header.
jpayne@69:
jpayne@69: Usage: comparessu.sh in= out=
jpayne@69:
jpayne@69: Input may be fasta or fastq, compressed or uncompressed.
jpayne@69:
jpayne@69: Standard parameters:
jpayne@69: in= Input sequences.
jpayne@69: out= Output data.
jpayne@69: t= Set the number of threads; default is logical processors.
jpayne@69: overwrite=f (ow) Set to false to force the program to abort rather than
jpayne@69: overwrite an existing file.
jpayne@69: showspeed=t (ss) Set to 'f' to suppress display of processing speed.
jpayne@69: ziplevel=2 (zl) Set to 1 (lowest) through 9 (max) to change compression
jpayne@69: level; lower compression is faster.
jpayne@69: reads=-1 If positive, quit after this many sequences.
jpayne@69:
jpayne@69: Processing parameters:
jpayne@69: ata=f Do an all-to-all comparison. Otherwise, each sequence will
jpayne@69: only be compared to one other randomly-selected sequence
jpayne@69: per taxonomic level.
jpayne@69: minlen=0 Ignore sequences shorter than this.
jpayne@69: maxlen=BIG Ignore sequences longer than this.
jpayne@69: maxns=-1 If positive, ignore sequences with more than this many Ns.
jpayne@69:
jpayne@69:
jpayne@69: Java Parameters:
jpayne@69: -Xmx This will set Java's memory usage, overriding autodetection.
jpayne@69: -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will
jpayne@69: specify 200 megs. The max is typically 85% of physical memory.
jpayne@69: -eoom This flag will cause the process to exit if an out-of-memory
jpayne@69: exception occurs. Requires Java 8u92+.
jpayne@69: -da Disable assertions.
jpayne@69:
jpayne@69: Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems.
jpayne@69: "
jpayne@69: }
jpayne@69:
jpayne@69: #This block allows symlinked shellscripts to correctly set classpath.
jpayne@69: pushd . > /dev/null
jpayne@69: DIR="${BASH_SOURCE[0]}"
jpayne@69: while [ -h "$DIR" ]; do
jpayne@69: cd "$(dirname "$DIR")"
jpayne@69: DIR="$(readlink "$(basename "$DIR")")"
jpayne@69: done
jpayne@69: cd "$(dirname "$DIR")"
jpayne@69: DIR="$(pwd)/"
jpayne@69: popd > /dev/null
jpayne@69:
jpayne@69: #DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/"
jpayne@69: CP="$DIR""current/"
jpayne@69:
jpayne@69: z="-Xmx4g"
jpayne@69: z2="-Xms4g"
jpayne@69: set=0
jpayne@69:
jpayne@69: if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then
jpayne@69: usage
jpayne@69: exit
jpayne@69: fi
jpayne@69:
jpayne@69: calcXmx () {
jpayne@69: source "$DIR""/calcmem.sh"
jpayne@69: setEnvironment
jpayne@69: parseXmx "$@"
jpayne@69: if [[ $set == 1 ]]; then
jpayne@69: return
jpayne@69: fi
jpayne@69: freeRam 4000m 84
jpayne@69: z="-Xmx${RAM}m"
jpayne@69: z2="-Xms${RAM}m"
jpayne@69: }
jpayne@69: calcXmx "$@"
jpayne@69:
jpayne@69: comparessu() {
jpayne@69: local CMD="java $EA $EOOM $z -cp $CP sketch.CompareSSU $@"
jpayne@69: echo $CMD >&2
jpayne@69: eval $CMD
jpayne@69: }
jpayne@69:
jpayne@69: comparessu "$@"