jpayne@69: #!/bin/bash jpayne@69: jpayne@69: usage(){ jpayne@69: echo " jpayne@69: Written by Brian Bushnell jpayne@69: Last modified April 30, 2019 jpayne@69: jpayne@69: Description: Performs set operations on VCF files: jpayne@69: Union, intersection, and subtraction. jpayne@69: jpayne@69: Usage: comparevcf.sh in= out= jpayne@69: jpayne@69: I/O parameters: jpayne@69: in= Input; must be at least 2 files. jpayne@69: out= Output file. jpayne@69: ref= Reference file; optional. Usually not needed. jpayne@69: shist= (scorehist) Output for variant score histogram. jpayne@69: overwrite=f (ow) Set to false to force the program to abort rather than jpayne@69: bgzip=f Use bgzip for gzip compression. jpayne@69: jpayne@69: Processing Mode (choose one only): jpayne@69: subtract=t Subtract all other files from the first file. jpayne@69: union=f Make a union of all files. jpayne@69: intersection=f Make an intersection of all files. jpayne@69: jpayne@69: Processing Parameters: jpayne@69: addsamples=t Include all samples in the output lines. (TODO) jpayne@69: splitalleles=f Split multi-allelic lines into multiple lines. jpayne@69: splitsubs=f Split multi-base substitutions into SNPs. jpayne@69: canonize=t Trim variations down to a canonical representation. jpayne@69: jpayne@69: Java Parameters: jpayne@69: -Xmx This will set Java's memory usage, overriding autodetection. jpayne@69: -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will jpayne@69: specify 200 megs. The max is typically 85% of physical memory. jpayne@69: -eoom This flag will cause the process to exit if an out-of-memory jpayne@69: exception occurs. Requires Java 8u92+. jpayne@69: -da Disable assertions. jpayne@69: jpayne@69: Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. jpayne@69: " jpayne@69: } jpayne@69: jpayne@69: #This block allows symlinked shellscripts to correctly set classpath. jpayne@69: pushd . > /dev/null jpayne@69: DIR="${BASH_SOURCE[0]}" jpayne@69: while [ -h "$DIR" ]; do jpayne@69: cd "$(dirname "$DIR")" jpayne@69: DIR="$(readlink "$(basename "$DIR")")" jpayne@69: done jpayne@69: cd "$(dirname "$DIR")" jpayne@69: DIR="$(pwd)/" jpayne@69: popd > /dev/null jpayne@69: jpayne@69: #DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" jpayne@69: CP="$DIR""current/" jpayne@69: jpayne@69: z="-Xmx4g" jpayne@69: z2="-Xms4g" jpayne@69: set=0 jpayne@69: jpayne@69: if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then jpayne@69: usage jpayne@69: exit jpayne@69: fi jpayne@69: jpayne@69: calcXmx () { jpayne@69: source "$DIR""/calcmem.sh" jpayne@69: setEnvironment jpayne@69: parseXmx "$@" jpayne@69: if [[ $set == 1 ]]; then jpayne@69: return jpayne@69: fi jpayne@69: freeRam 4000m 84 jpayne@69: z="-Xmx${RAM}m" jpayne@69: z2="-Xms${RAM}m" jpayne@69: } jpayne@69: calcXmx "$@" jpayne@69: jpayne@69: comparevcf() { jpayne@69: local CMD="java $EA $EOOM $z $z2 -cp $CP var2.CompareVCF $@" jpayne@69: echo $CMD >&2 jpayne@69: eval $CMD jpayne@69: } jpayne@69: jpayne@69: comparevcf "$@"