jpayne@69: #!/bin/bash jpayne@69: jpayne@69: usage(){ jpayne@69: echo " jpayne@69: Written by Brian Bushnell jpayne@69: Last modified October 3, 2017 jpayne@69: jpayne@69: Description: Creates polymer sequences. jpayne@69: Can be used in conjunction with mutate.sh to generate low-complexity sequence. jpayne@69: jpayne@69: Usage: makepolymers.sh out= k= minlen= jpayne@69: jpayne@69: I/O parameters: jpayne@69: out= Output genome. jpayne@69: overwrite=f (ow) Set to false to force the program to abort rather than jpayne@69: overwrite an existing file. jpayne@69: jpayne@69: Processing parameters: jpayne@69: k=1 Length of repeating polymeric units. jpayne@69: To generate a sweep of multiple values of k, jpayne@69: specify both mink and maxk. jpayne@69: minlen=31 Ensure sequences are at least this long. jpayne@69: Specifically, minlen=X will ensure sequences are long enough jpayne@69: that all possible kmers of length X are present. jpayne@69: jpayne@69: Java Parameters: jpayne@69: -Xmx This will set Java's memory usage, overriding autodetection. jpayne@69: -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will jpayne@69: specify 200 megs. The max is typically 85% of physical memory. jpayne@69: -eoom This flag will cause the process to exit if an out-of-memory jpayne@69: exception occurs. Requires Java 8u92+. jpayne@69: -da Disable assertions. jpayne@69: jpayne@69: Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. jpayne@69: " jpayne@69: } jpayne@69: jpayne@69: #This block allows symlinked shellscripts to correctly set classpath. jpayne@69: pushd . > /dev/null jpayne@69: DIR="${BASH_SOURCE[0]}" jpayne@69: while [ -h "$DIR" ]; do jpayne@69: cd "$(dirname "$DIR")" jpayne@69: DIR="$(readlink "$(basename "$DIR")")" jpayne@69: done jpayne@69: cd "$(dirname "$DIR")" jpayne@69: DIR="$(pwd)/" jpayne@69: popd > /dev/null jpayne@69: jpayne@69: #DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" jpayne@69: CP="$DIR""current/" jpayne@69: jpayne@69: z="-Xmx4g" jpayne@69: z2="-Xms4g" jpayne@69: set=0 jpayne@69: jpayne@69: if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then jpayne@69: usage jpayne@69: exit jpayne@69: fi jpayne@69: jpayne@69: calcXmx () { jpayne@69: source "$DIR""/calcmem.sh" jpayne@69: setEnvironment jpayne@69: parseXmx "$@" jpayne@69: if [[ $set == 1 ]]; then jpayne@69: return jpayne@69: fi jpayne@69: freeRam 4000m 84 jpayne@69: z="-Xmx${RAM}m" jpayne@69: z2="-Xms${RAM}m" jpayne@69: } jpayne@69: calcXmx "$@" jpayne@69: jpayne@69: makepolymers() { jpayne@69: local CMD="java $EA $EOOM $z $z2 -cp $CP jgi.MakePolymers $@" jpayne@69: echo $CMD >&2 jpayne@69: eval $CMD jpayne@69: } jpayne@69: jpayne@69: makepolymers "$@"