jpayne@69: #!/bin/bash jpayne@69: jpayne@69: usage(){ jpayne@69: echo " jpayne@69: Written by Brian Bushnell jpayne@69: Last modified December 19, 2019 jpayne@69: jpayne@69: Description: Merges multiple sketches into a single sketch. jpayne@69: jpayne@69: Please read bbmap/docs/guides/BBSketchGuide.txt for more information. jpayne@69: jpayne@69: Usage: mergesketch.sh in=a.sketch,b.sketch out=c.sketch jpayne@69: With wildcards: mergesketch.sh *.sketch out=c.sketch jpayne@69: jpayne@69: Standard parameters: jpayne@69: in= Input sketches or fasta files; may be a comma-delimited jpayne@69: list. in= is optional so wildcards may be used. jpayne@69: out= Output sketch. jpayne@69: amino=f Use amino acid mode. jpayne@69: jpayne@69: Sketch-making parameters: jpayne@69: mode=single Possible modes, for fasta input: jpayne@69: single: Generate one sketch per file. jpayne@69: sequence: Generate one sketch per sequence. jpayne@69: autosize=t Produce an output sketch of whatever size the union jpayne@69: happens to be. jpayne@69: size= Restrict output sketch to this upper bound of size. jpayne@69: k=32,24 Kmer length, 1-32. jpayne@69: keyfraction=0.2 Only consider this upper fraction of keyspace. jpayne@69: minkeycount=1 Ignore kmers that occur fewer times than this. Values jpayne@69: over 1 can be used with raw reads to avoid error kmers. jpayne@69: depth=f Retain kmer counts if available. jpayne@69: jpayne@69: Metadata parameters: (if blank the values of the first sketch will be used) jpayne@69: taxid=-1 Set the NCBI taxid. jpayne@69: imgid=-1 Set the IMG id. jpayne@69: spid=-1 Set the JGI sequencing project id. jpayne@69: name= Set the name (taxname). jpayne@69: name0= Set name0 (normally the first sequence header). jpayne@69: fname= Set fname (normally the file name). jpayne@69: meta_= Set an arbitrary metadata field. jpayne@69: For example, meta_Month=March. jpayne@69: jpayne@69: Java Parameters: jpayne@69: -Xmx This will set Java's memory usage, overriding autodetection. jpayne@69: -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will specify 200 megs. jpayne@69: The max is typically 85% of physical memory. jpayne@69: -eoom This flag will cause the process to exit if an out-of-memory jpayne@69: exception occurs. Requires Java 8u92+. jpayne@69: -da Disable assertions. jpayne@69: jpayne@69: For more detailed information, please read /bbmap/docs/guides/BBSketchGuide.txt. jpayne@69: Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. jpayne@69: " jpayne@69: } jpayne@69: jpayne@69: #This block allows symlinked shellscripts to correctly set classpath. jpayne@69: pushd . > /dev/null jpayne@69: DIR="${BASH_SOURCE[0]}" jpayne@69: while [ -h "$DIR" ]; do jpayne@69: cd "$(dirname "$DIR")" jpayne@69: DIR="$(readlink "$(basename "$DIR")")" jpayne@69: done jpayne@69: cd "$(dirname "$DIR")" jpayne@69: DIR="$(pwd)/" jpayne@69: popd > /dev/null jpayne@69: jpayne@69: #DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" jpayne@69: CP="$DIR""current/" jpayne@69: jpayne@69: z="-Xmx4g" jpayne@69: z2="-Xms4g" jpayne@69: set=0 jpayne@69: jpayne@69: if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then jpayne@69: usage jpayne@69: exit jpayne@69: fi jpayne@69: jpayne@69: calcXmx () { jpayne@69: source "$DIR""/calcmem.sh" jpayne@69: setEnvironment jpayne@69: parseXmx "$@" jpayne@69: if [[ $set == 1 ]]; then jpayne@69: return jpayne@69: fi jpayne@69: freeRam 3200m 84 jpayne@69: z="-Xmx${RAM}m" jpayne@69: z2="-Xms${RAM}m" jpayne@69: } jpayne@69: calcXmx "$@" jpayne@69: jpayne@69: sendsketch() { jpayne@69: local CMD="java $EA $EOOM $z -cp $CP sketch.MergeSketch $@" jpayne@69: # echo $CMD >&2 jpayne@69: eval $CMD jpayne@69: } jpayne@69: jpayne@69: sendsketch "$@"