jpayne@69: #!/bin/bash jpayne@69: jpayne@69: usage(){ jpayne@69: echo " jpayne@69: Written by Brian Bushnell jpayne@69: Last modified December 19, 2018 jpayne@69: Description: Generates a length histogram of input reads. jpayne@69: jpayne@69: Usage: readlength.sh in= jpayne@69: jpayne@69: in= The 'in=' flag is needed only if the input file is not the first parameter. 'in=stdin.fq' will pipe from standard in. jpayne@69: in2= Use this if 2nd read of pairs are in a different file. jpayne@69: out= Write the histogram to this file. Default is stdout. jpayne@69: bin=10 Set the histogram bin size. jpayne@69: max=80000 Set the max read length to track. jpayne@69: round=f Places reads in the closest bin, rather than the highest bin of at least readlength. jpayne@69: nzo=t (nonzeroonly) Do not print empty bins. jpayne@69: reads=-1 If nonnegative, stop after this many reads. jpayne@69: jpayne@69: Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. jpayne@69: " jpayne@69: } jpayne@69: jpayne@69: #This block allows symlinked shellscripts to correctly set classpath. jpayne@69: pushd . > /dev/null jpayne@69: DIR="${BASH_SOURCE[0]}" jpayne@69: while [ -h "$DIR" ]; do jpayne@69: cd "$(dirname "$DIR")" jpayne@69: DIR="$(readlink "$(basename "$DIR")")" jpayne@69: done jpayne@69: cd "$(dirname "$DIR")" jpayne@69: DIR="$(pwd)/" jpayne@69: popd > /dev/null jpayne@69: jpayne@69: #DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" jpayne@69: CP="$DIR""current/" jpayne@69: jpayne@69: z="-Xmx400m" jpayne@69: z2="-Xms400m" jpayne@69: set=0 jpayne@69: jpayne@69: if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then jpayne@69: usage jpayne@69: exit jpayne@69: fi jpayne@69: jpayne@69: calcXmx () { jpayne@69: source "$DIR""/calcmem.sh" jpayne@69: setEnvironment jpayne@69: parseXmx "$@" jpayne@69: } jpayne@69: calcXmx "$@" jpayne@69: jpayne@69: stats() { jpayne@69: local CMD="java $EA $EOOM $z -cp $CP jgi.MakeLengthHistogram $@" jpayne@69: # echo $CMD >&2 jpayne@69: eval $CMD jpayne@69: } jpayne@69: jpayne@69: stats "$@"