annotate aws_sra.xml @ 21:02f45c03c306 draft

planemo upload for repository https://github.com/CFSAN-Biostatistics/galaxytrakr-tools commit f72940592c22c9ba88f1dcb23ef8bb5199ce434e
author galaxytrakr
date Mon, 23 Mar 2026 23:33:12 +0000
parents 2b4efa539c71
children 5ecb94ab82c3
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1 <tool id="aws_sra" name="NCBI SRA AWS Fetch" version="1.3.0" profile="23.0">
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2 <description>Fetches a single SRA run from AWS and converts it to FASTQ</description>
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4 <requirements>
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5 <requirement type="package" version="2.34.8">awscli</requirement>
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6 <requirement type="package" version="3.2.1">sra-tools</requirement>
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7 <requirement type="package" version="2.8">pigz</requirement>
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8 </requirements>
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10 <version_command>fasterq-dump --version</version_command>
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12 <command detect_errors="aggressive"><![CDATA[
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13 #set $acc = str($accession).strip()
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15 echo "Processing single accession: $acc" &&
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17 ## 1. Create temporary directories
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18 mkdir -p sra_cache fastq_out &&
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20 ## 2. Download the file from S3 using the discovered path format (no .sra)
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21 aws s3 cp --no-sign-request 's3://sra-pub-run-odp/sra/${acc}/${acc}' ./sra_cache/ &&
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23 ## 3. Convert with fasterq-dump, using the correct argument order
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24 fasterq-dump --outdir ./fastq_out --temp . --threads \${GALAXY_SLOTS:-4} --split-files ./sra_cache/${acc} &&
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26 ## 4. Compress with pigz
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27 pigz -p \${GALAXY_SLOTS:-4} ./fastq_out/*.fastq &&
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29 ## 5. Move the final outputs to their Galaxy dataset paths
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30 #if str($layout) == 'paired'
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31 mv ./fastq_out/${acc}_1.fastq.gz '$output_r1' &&
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32 mv ./fastq_out/${acc}_2.fastq.gz '$output_r2'
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33 #else
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34 # Be explicit about the single-end filename, removing the wildcard
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35 mv ./fastq_out/${acc}.fastq.gz '$output_r1'
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36 #end if
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37 ]]></command>
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39 <inputs>
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40 <param name="accession" type="text" label="SRA Accession" help="e.g., SRR13333333"/>
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41 <param name="layout" type="select" label="Read layout" help="Check the SRA record to confirm layout before running.">
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42 <option value="paired" selected="true">Paired-end (R1 + R2)</option>
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43 <option value="single">Single-end</option>
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44 </param>
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45 </inputs>
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47 <outputs>
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48 <data name="output_r1" format="fastqsanger.gz" label="${accession}_1.fastq.gz"/>
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49 <data name="output_r2" format="fastqsanger.gz" label="${accession}_2.fastq.gz">
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50 <filter>layout == 'paired'</filter>
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51 </data>
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52 </outputs>
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53
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54 <tests>
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55 <test expect_num_outputs="2">
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56 <param name="accession" value="SRR13333333"/>
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57 <param name="layout" value="paired"/>
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58 <output name="output_r1" ftype="fastqsanger.gz">
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59 <assert_contents>
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60 <has_text text="@SRR13333333"/>
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61 </assert_contents>
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62 </output>
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63 <output name="output_r2" ftype="fastqsanger.gz">
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64 <assert_contents>
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65 <has_text text="@SRR13333333"/>
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66 </assert_contents>
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67 </output>
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68 </test>
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69 <test expect_num_outputs="1">
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70 <param name="accession" value="SRR11181815"/> <!-- A known single-end run -->
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71 <param name="layout" value="single"/>
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72 <output name="output_r1" ftype="fastqsanger.gz">
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73 <assert_contents>
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74 <has_text text="@SRR11181815"/>
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75 </assert_contents>
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76 </output>
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77 </test>
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78 </tests>
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79
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80 <help><![CDATA[
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81 **NCBI SRA AWS Fetch**
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82
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83 Fetches a single SRA run from the public `sra-pub-run-odp` bucket on Amazon S3 and converts it to gzip-compressed FASTQ using `fasterq-dump`.
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84 ]]></help>
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85
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86 <citations>
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87 <citation type="bibtex">
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88 @misc{ncbi_sra_aws,
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89 title = {{NCBI} {SRA} on {AWS} Open Data},
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90 author = {{National Center for Biotechnology Information}},
13
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91 howpublished = {\\url{https://registry.opendata.aws/ncbi-sra/}},
0
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92 note = {Accessed via AWS S3 without credentials}
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93 }
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94 </citation>
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95 <citation type="bibtex">
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96 @article{sra_toolkit,
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97 title = {The {NCBI} {SRA} and portable data in biology},
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98 author = {Leinonen, Rasko and Sugawara, Hideaki and Shumway, Martin and
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99 {International Nucleotide Sequence Database Collaboration}},
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100 journal = {Nucleic Acids Research},
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101 volume = {39},
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102 number = {suppl\\\_1},
0
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103 pages = {D19--D21},
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104 year = {2011},
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105 doi = {10.1093/nar/gkq1019}
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106 }
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107 </citation>
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108 </citations>
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109 </tool>