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kkonganti@1
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1 // Help text for mash sketch within CPIPES.
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2
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kkonganti@1
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3 def mashsketchHelp(params) {
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4
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5 Map tool = [:]
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6 Map toolspecs = [:]
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7 tool.text = [:]
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8 tool.helpparams = [:]
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9
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10 toolspecs = [
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11 'mashsketch_run': [
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12 clihelp: 'Run `mash screen` tool. Default: ' +
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13 (params.mashsketch_run ?: false),
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14 cliflag: null,
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15 clivalue: null
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16 ],
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17 'mashsketch_l': [
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18 clihelp: 'List input. Lines in each <input> specify paths to sequence files, ' +
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19 'one per line. ' +
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20 "Default: ${params.mashsketch_l}",
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21 cliflag: '-l',
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22 clivalue: (params.mashsketch_l ? ' ' : '')
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23 ],
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24 'mashsketch_I': [
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25 clihelp: '<path> ID field for sketch of reads (instead of first sequence ID). ' +
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26 "Default: ${params.mashsketch_I}",
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27 cliflag: '-I',
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28 clivalue: (params.mashsketch_I ?: '')
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29 ],
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30 'mashsketch_C': [
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31 clihelp: '<path> Comment for a sketch of reads (instead of first sequence comment). ' +
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32 "Default: ${params.mashsketch_C}",
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33 cliflag: '-C',
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34 clivalue: (params.mashsketch_C ?: '')
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35 ],
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36 'mashsketch_k': [
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37 clihelp: '<int> K-mer size. Hashes will be based on strings of this many ' +
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kkonganti@1
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38 'nucleotides. Canonical nucleotides are used by default (see ' +
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39 'Alphabet options below). (1-32) ' +
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40 "Default: ${params.mashsketch_k}",
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41 cliflag: '-k',
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42 clivalue: (params.mashsketch_k ?: '')
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43 ],
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44 'mashsketch_s': [
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45 clihelp: '<int> Sketch size. Each sketch will have at most this many non-redundant ' +
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46 'min-hashes. ' +
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47 "Default: ${params.mashsketch_s}",
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48 cliflag: '-s',
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49 clivalue: (params.mashsketch_s ?: '')
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50 ],
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51 'mashsketch_i': [
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52 clihelp: 'Sketch individual sequences, rather than whole files, e.g. for ' +
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53 'multi-fastas of single-chromosome genomes or pair-wise gene ' +
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54 'comparisons. ' +
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55 "Default: ${params.mashsketch_i}",
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56 cliflag: '-i',
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57 clivalue: (params.mashsketch_i ? ' ' : '')
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58 ],
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59 'mashsketch_S': [
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60 clihelp: '<int> Seed to provide to the hash function. (0-4294967296) [42] ' +
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61 "Default: ${params.mashsketch_S}",
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62 cliflag: '-S',
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63 clivalue: (params.mashsketch_S ?: '')
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64 ],
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65
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66 'mashsketch_w': [
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67 clihelp: '<num> Probability threshold for warning about low k-mer size. (0-1) ' +
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68 "Default: ${params.mashsketch_w}",
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69 cliflag: '-w',
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70 clivalue: (params.mashsketch_w ?: '')
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71 ],
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72 'mashsketch_r': [
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73 clihelp: 'Input is a read set. See Reads options below. Incompatible with ' +
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74 '--mashsketch_i. ' +
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75 "Default: ${params.mashsketch_r}",
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76 cliflag: '-r',
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77 clivalue: (params.mashsketch_r ? ' ' : '')
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78 ],
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79 'mashsketch_b': [
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80 clihelp: '<size> Use a Bloom filter of this size (raw bytes or with K/M/G/T) to ' +
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81 'filter out unique k-mers. This is useful if exact filtering with ' +
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82 '--mashsketch_m uses too much memory. However, some unique k-mers may pass ' +
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83 'erroneously, and copies cannot be counted beyond 2. Implies --mashsketch_r. ' +
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84 "Default: ${params.mashsketch_b}",
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85 cliflag: '-b',
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86 clivalue: (params.mashsketch_b ?: '')
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87 ],
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88 'mashsketch_m': [
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89 clihelp: '<int> Minimum copies of each k-mer required to pass noise filter for ' +
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90 'reads. Implies --mashsketch_r. ' +
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91 "Default: ${params.mashsketch_r}",
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92 cliflag: '-m',
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93 clivalue: (params.mashsketch_m ?: '')
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94 ],
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95 'mashsketch_c': [
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96 clihelp: '<num> Target coverage. Sketching will conclude if this coverage is ' +
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97 'reached before the end of the input file (estimated by average ' +
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98 'k-mer multiplicity). Implies --mashsketch_r. ' +
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99 "Default: ${params.mashsketch_c}",
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100 cliflag: '-c',
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101 clivalue: (params.mashsketch_c ?: '')
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102 ],
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103 'mashsketch_g': [
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104 clihelp: '<size> Genome size (raw bases or with K/M/G/T). If specified, will be used ' +
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105 'for p-value calculation instead of an estimated size from k-mer ' +
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106 'content. Implies --mashsketch_r. ' +
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107 "Default: ${params.mashsketch_g}",
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108 cliflag: '-g',
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109 clivalue: (params.mashsketch_g ?: '')
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110 ],
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111 'mashsketch_n': [
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112 clihelp: 'Preserve strand (by default, strand is ignored by using canonical ' +
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113 'DNA k-mers, which are alphabetical minima of forward-reverse ' +
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114 'pairs). Implied if an alphabet is specified with --mashsketch_a ' +
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115 'or --mashsketch_z. ' +
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116 "Default: ${params.mashsketch_n}",
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117 cliflag: '-n',
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118 clivalue: (params.mashsketch_n ? ' ' : '')
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119 ],
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120 'mashsketch_a': [
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121 clihelp: 'Use amino acid alphabet (A-Z, except BJOUXZ). Implies ' +
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122 '--mashsketch_n --mashsketch_k 9. ' +
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123 "Default: ${params.mashsketch_a}",
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124 cliflag: '-a',
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125 clivalue: (params.mashsketch_a ? ' ' : '')
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126 ],
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127 'mashsketch_z': [
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128 clihelp: '<text> Alphabet to base hashes on (case ignored by default; ' +
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129 'see --mashsketch_Z). K-mers with other characters will be ' +
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130 'ignored. Implies --mashsketch_n. ' +
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131 "Default: ${params.mashsketch_z}",
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132 cliflag: '-z',
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133 clivalue: (params.mashsketch_z ?: '')
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134 ],
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135 'mashsketch_Z': [
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136 clihelp: 'Preserve case in k-mers and alphabet (case is ignored by default). ' +
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137 'Sequence letters whose case is not in the current alphabet will be ' +
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138 'skipped when sketching. ' +
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139 "Default: ${params.mashsketch_Z}",
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140 cliflag: '-Z',
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141 clivalue: (params.mashsketch_Z ?: '')
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142 ]
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143 ]
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144
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145 toolspecs.each {
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146 k, v -> tool.text['--' + k] = "${v.clihelp}"
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147 tool.helpparams[k] = [ cliflag: "${v.cliflag}", clivalue: v.clivalue ]
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148 }
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149
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150 return tool
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151 } |
