Mercurial > repos > kkonganti > cfsan_bettercallsal
view 0.7.0/subworkflows/process_fastq.nf @ 21:4ce0e079377d tip
planemo upload
author | kkonganti |
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date | Mon, 15 Jul 2024 12:01:00 -0400 |
parents | 0e7a0053e4a6 |
children |
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// Include any necessary methods and modules include { stopNow; validateParamsForFASTQ } from "${params.routines}" include { GEN_SAMPLESHEET } from "${params.modules}${params.fs}gen_samplesheet${params.fs}main" include { SAMPLESHEET_CHECK } from "${params.modules}${params.fs}samplesheet_check${params.fs}main" include { CAT_FASTQ } from "${params.modules}${params.fs}cat${params.fs}fastq${params.fs}main" include { SEQKIT_SEQ } from "${params.modules}${params.fs}seqkit${params.fs}seq${params.fs}main" // Validate 4 required workflow parameters if // FASTQ files are the input for the // entry point. validateParamsForFASTQ() // Start the subworkflow workflow PROCESS_FASTQ { main: versions = Channel.empty() input_ch = Channel.empty() reads = Channel.empty() def input = file( (params.input ?: params.metadata) ) if (params.input) { def fastq_files = [] if (params.fq_suffix == null) { stopNow("We need to know what suffix the FASTQ files ends with inside the\n" + "directory. Please use the --fq_suffix option to indicate the file\n" + "suffix by which the files are to be collected to run the pipeline on.") } if (params.fq_strandedness == null) { stopNow("We need to know if the FASTQ files inside the directory\n" + "are sequenced using stranded or non-stranded sequencing. This is generally\n" + "required if the sequencing experiment is RNA-SEQ. For almost all of the other\n" + "cases, you can probably use the --fq_strandedness unstranded option to indicate\n" + "that the reads are unstranded.") } if (params.fq_filename_delim == null || params.fq_filename_delim_idx == null) { stopNow("We need to know the delimiter of the filename of the FASTQ files.\n" + "By default the filename delimiter is _ (underscore). This delimiter character\n" + "is used to split and assign a group name. The group name can be controlled by\n" + "using the --fq_filename_delim_idx option (1-based). For example, if the FASTQ\n" + "filename is WT_REP1_001.fastq, then to create a group WT, use the following\n" + "options: --fq_filename_delim _ --fq_filename_delim_idx 1") } if (!input.exists()) { stopNow("The input directory,\n${params.input}\ndoes not exist!") } input.eachFileRecurse { it.name.endsWith("${params.fq_suffix}") ? fastq_files << it : fastq_files << null } if (fastq_files.findAll{ it != null }.size() == 0) { stopNow("The input directory,\n${params.input}\nis empty! or does not " + "have FASTQ files ending with the suffix: ${params.fq_suffix}") } GEN_SAMPLESHEET( Channel.fromPath(params.input, type: 'dir') ) GEN_SAMPLESHEET.out.csv.set{ input_ch } versions.mix( GEN_SAMPLESHEET.out.versions ) .set { versions } } else if (params.metadata) { if (!input.exists()) { stopNow("The metadata CSV file,\n${params.metadata}\ndoes not exist!") } if (input.size() <= 0) { stopNow("The metadata CSV file,\n${params.metadata}\nis empty!") } Channel.fromPath(params.metadata, type: 'file') .set { input_ch } } SAMPLESHEET_CHECK( input_ch ) .csv .splitCsv( header: true, sep: ',') .map { create_fastq_channel(it) } .groupTuple(by: [0]) .branch { meta, fastq -> single : fastq.size() == 1 return [ meta, fastq.flatten() ] multiple : fastq.size() > 1 return [ meta, fastq.flatten() ] } .set { reads } CAT_FASTQ( reads.multiple ) .catted_reads .mix( reads.single ) .set { processed_reads } if (params.fq_filter_by_len.toInteger() > 0) { SEQKIT_SEQ( processed_reads ) .fastx .set { processed_reads } versions.mix( SEQKIT_SEQ.out.versions.first().ifEmpty(null) ) .set { versions } } versions.mix( SAMPLESHEET_CHECK.out.versions, CAT_FASTQ.out.versions.first().ifEmpty(null) ) .set { versions } emit: processed_reads versions } // Function to get list of [ meta, [ fq1, fq2 ] ] def create_fastq_channel(LinkedHashMap row) { def meta = [:] meta.id = row.sample meta.single_end = row.single_end.toBoolean() meta.strandedness = row.strandedness meta.id = meta.id.split(params.fq_filename_delim)[0..params.fq_filename_delim_idx.toInteger() - 1] .join(params.fq_filename_delim) meta.id = (meta.id =~ /\./ ? meta.id.take(meta.id.indexOf('.')) : meta.id) def array = [] if (!file(row.fq1).exists()) { stopNow("Please check input metadata CSV. The following Read 1 FASTQ file does not exist!" + "\n${row.fq1}") } if (meta.single_end) { array = [ meta, [ file(row.fq1) ] ] } else { if (!file(row.fq2).exists()) { stopNow("Please check input metadata CSV. The following Read 2 FASTQ file does not exist!" + "\n${row.fq2}") } array = [ meta, [ file(row.fq1), file(row.fq2) ] ] } return array }