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1 package tax;
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2
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3 import java.io.File;
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4 import java.util.ArrayList;
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5
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6 import fileIO.ByteFile;
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7 import fileIO.ReadWrite;
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8 import shared.Parse;
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9 import shared.Shared;
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10 import shared.Tools;
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11 import structures.IntList;
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12
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13 /**
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14 * @author Brian Bushnell
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15 * @date Mar 10, 2015
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16 *
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17 */
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18 public class GiToTaxidInt {
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19
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20 public static void main(String[] args){
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21 ReadWrite.USE_UNPIGZ=true;
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22 ReadWrite.USE_PIGZ=true;
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23 ReadWrite.ZIPLEVEL=9;
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24 ReadWrite.PIGZ_BLOCKSIZE=256;
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25 // ReadWrite.PIGZ_ITERATIONS=30;
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26
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27 for(String arg : args){
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28 String[] split=arg.split("=");
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29 String a=split[0].toLowerCase();
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30 String b=split.length>1 ? split[1] : null;
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31 shared.Parser.parseZip(arg, a, b);
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32 }
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33 // if(args.length>2 && false){//Run a test
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34 // test(args);
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35 // }else
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36 if(args.length>=2){//Write array
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37 initialize(args[0]);
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38 ReadWrite.write(array, args[1], true);
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39 }
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40 }
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41
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42 public static void test(String[] args){
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43 System.err.println(getID(1000));
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44 System.err.println(getID(10000));
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45 System.err.println(getID(10001));
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46 System.err.println(getID(10002));
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47 System.err.println(getID(10003));
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48 System.err.println(getID(10004));
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49 System.err.println(getID(10005));
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50 System.err.println(getID(100000));
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51 System.err.println(getID(1000000));
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52 System.err.println(getID(10000000));
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53
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54 TaxTree tree=null;
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55 if(args.length>1){
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56 tree=TaxTree.loadTaxTree(args[0], System.err, true, true);
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57 }
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58
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59 System.err.println("Strings:");
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60 int x;
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61 x=getID("gi|18104025|emb|AJ427095.1| Ceratitis capitata centromeric or pericentromeric satellite DNA, clone 44");
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62 System.err.println(x);
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63 if(tree!=null){
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64 System.err.println(tree.getNode(x));
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65 tree.incrementRaw(x, 30);
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66 }
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67 x=getID("gi|15982920|gb|AY057568.1| Arabidopsis thaliana AT5g43500/MWF20_22 mRNA, complete cds");
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68 System.err.println(x);
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69 if(tree!=null){
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70 System.err.println(tree.getNode(x));
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71 tree.incrementRaw(x, 40);
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72 }
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73 x=getID("gi|481043749|gb|KC494054.1| Plesiochorus cymbiformis isolate ST05-58 internal transcribed spacer 2, partial sequence");
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74 System.err.println(x);
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75 if(tree!=null){
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76 System.err.println(tree.getNode(x));
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77 tree.incrementRaw(x, 20);
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78 }
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79
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80 if(tree!=null){
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81 tree.percolateUp();
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82 ArrayList<TaxNode> nodes=tree.gatherNodesAtLeastLimit(35);
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83 for(TaxNode n : nodes){
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84 System.err.println(n);
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85 }
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86 }
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87 }
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88
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89 public static int parseGiToTaxid(String s){return parseGiToTaxid(s, '|');}
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90 public static int parseGiToTaxid(String s, char delimiter){
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91 int x=parseGiNumber(s, delimiter);
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92 assert(x>=0) : s;
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93 assert(array!=null) : "To use gi numbers, you must load a gi table.";
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94 // if(x>=array.length || array[x]<0){x=(int)(Math.random()*array.length);} //Test to make sure array is nonempty.
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95 if(x>=0 && x<array.length){return array[x];}
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96 assert(x<array.length) : "The GI number "+x+" is too big.\n"
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97 + "Please update the gi table with the latest version from NCBI as per the instructions in gitable.sh.\n"
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98 + "To ignore this problem, please run with the -da flag.\n";
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99 return -1;
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100 }
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101
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102
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103 public static int parseGiToTaxid(byte[] s){return parseGiToTaxid(s, '|');}
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104 public static int parseGiToTaxid(byte[] s, char delimiter){
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105 long x=parseGiNumber(s, delimiter);
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106 if(x>=0 && x<array.length){return array[(int)x];}
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107 if(x<0){return -1;}
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108 assert(false) : x;
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109 return -1;
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110 }
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111
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112 /** Parse a gi number, or return -1 if formatted incorrectly. */
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113 static int parseGiNumber(String s, char delimiter){
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114 if(s==null || s.length()<4){return -1;}
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115 // System.err.println("a");
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116 if(s.charAt(0)=='>'){return getID(s.substring(1), delimiter);}
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117 // System.err.println("b");
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118 if(!s.startsWith("gi")){return -1;}
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119 // System.err.println("c");
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120 // System.err.println("d");
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121 int initial=s.indexOf(delimiter);
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122 // System.err.println("e");
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123 if(initial<0){
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124 if(delimiter!='~'){
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125 delimiter='~';
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126 initial=s.indexOf(delimiter);
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127 }
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128 if(initial<0){
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129 delimiter='_';
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130 initial=s.indexOf(delimiter);
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131 }
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132 if(initial<0){return -1;}
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133 // System.err.println("f");
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134 // System.err.println("g");
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135 }
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jpayne@68
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136 // System.err.println("h");
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137 if(!Tools.isDigit(s.charAt(initial+1))){return -1;}
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138 // System.err.println("i");
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139
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140 int number=0;
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141 for(int i=initial+1; i<s.length(); i++){
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142 char c=s.charAt(i);
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143 if(c==delimiter){break;}
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144 assert(Tools.isDigit(c));
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145 number=(number*10)+(c-'0');
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146 }
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147 // System.err.println("j: "+number);
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148 return number;
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149 }
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150
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151 /** Parse a ncbi number, or return -1 if formatted incorrectly. */
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152 public static int parseTaxidNumber(String s, char delimiter){
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153 if(s==null || s.length()<5){return -1;}
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154 if(s.charAt(0)=='>'){return parseTaxidNumber(s.substring(1), delimiter);}
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155 if(!s.startsWith("ncbi") && !s.startsWith("tid")){return -1;}
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156 int initial=s.indexOf(delimiter);
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157 if(initial<0){
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158 delimiter='_';
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159 initial=s.indexOf(delimiter);
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160 if(initial<0){return -1;}
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161 }
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162 if(!Tools.isDigit(s.charAt(initial+1))){return -1;}
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163
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164 int number=0;
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165 for(int i=initial+1; i<s.length(); i++){
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166 char c=s.charAt(i);
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167 if(c==delimiter || c==' '){break;}
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168 assert(Tools.isDigit(c)) : c+"\n"+s;
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169 number=(number*10)+(c-'0');
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170 }
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171 return number;
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172 }
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173
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174
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175 public static int getID(String s){return getID(s, '|');}
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jpayne@68
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176 /** Get the taxID from a header starting with a taxID or gi number */
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177 public static int getID(String s, char delimiter){
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178 int x=parseTaxidNumber(s, delimiter);
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179 if(x>=0){return x;}
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180 x=parseGiNumber(s, delimiter);
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181 if(x>=0){return array[x];}
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182 return -1;
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183 }
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184
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185 /** Parse a gi number, or return -1 if formatted incorrectly. */
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186 static int parseGiNumber(byte[] s, char delimiter){
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187 if(s==null || s.length<4){return -1;}
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188 if(!Tools.startsWith(s, "gi") && !Tools.startsWith(s, ">gi")){return -1;}
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189 int initial=Tools.indexOf(s, (byte)delimiter);
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190 if(initial<0){
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191 delimiter='_';
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192 initial=Tools.indexOf(s, (byte)delimiter);
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193 if(initial<0){return -1;}
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194 }
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195 if(!Tools.isDigit(s[initial+1])){return -1;}
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196
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197 long number=0;
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198 for(int i=initial+1; i<s.length; i++){
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199 byte c=s[i];
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200 if(c==delimiter){break;}
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201 assert(Tools.isDigit(c));
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202 number=(number*10)+(c-'0');
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203 }
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204 return (int)number;
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205 }
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206
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207 /** Parse a gi number, or return -1 if formatted incorrectly. */
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208 static int parseNcbiNumber(byte[] s, char delimiter){
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209 if(s==null || s.length<3){return -1;}
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210 if(!Tools.startsWith(s, "ncbi") && !Tools.startsWith(s, ">ncbi") && !Tools.startsWith(s, "tid") && !Tools.startsWith(s, ">tid")){return -1;}
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211 int initial=Tools.indexOf(s, (byte)delimiter);
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212 if(initial<0){
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213 delimiter='_';
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214 initial=Tools.indexOf(s, (byte)delimiter);
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215 if(initial<0){return -1;}
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216 }
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jpayne@68
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217 if(!Tools.isDigit(s[initial+1])){return -1;}
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218
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219 int number=0;
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220 for(int i=initial+1; i<s.length; i++){
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221 byte c=s[i];
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222 if(c==delimiter){break;}
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223 assert(Tools.isDigit(c));
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224 number=(number*10)+(c-'0');
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225 }
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jpayne@68
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226 return number;
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227 }
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228
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229 public static int getID(byte[] s){return getID(s, '|');}
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jpayne@68
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230 /** Get the taxID from a header starting with a taxID or gi number */
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231 public static int getID(byte[] s, char delimiter){
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232 int x=parseGiNumber(s, delimiter);
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233 if(x>=0){return array[x];}
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234 return parseNcbiNumber(s, delimiter);
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jpayne@68
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235 }
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236
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jpayne@68
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237 /** Get the taxID from a gi number */
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238 public static int getID(long gi){
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239 assert(gi>=0) : gi;
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240 assert(gi<Integer.MAX_VALUE) : gi+" > "+Integer.MAX_VALUE;
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241 assert(gi<array.length) : gi+", "+array.length;
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242 return array[(int)gi];
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jpayne@68
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243 }
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244
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jpayne@68
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245 public static void initialize(String fname){
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246 assert(fname!=null);
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247 if(fileString==null || !fileString.equals(fname)){
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248 synchronized(GiToTaxid.class){
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249 if(!initialized || fileString==null || !fileString.equals(fname)){
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250 fileString=fname;
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251 if(fname.contains(".int1d")){
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252 array=ReadWrite.read(int[].class, fname, true);
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253 }else{
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254 array=makeArray(fname);
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255 }
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jpayne@68
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256 }
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257 initialized=true;
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258 }
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jpayne@68
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259 }
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jpayne@68
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260 }
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261
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jpayne@68
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262 public static boolean isInitialized(){return initialized;}
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263
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264 public static synchronized void unload(){
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265 array=null;
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266 fileString=null;
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267 initialized=false;
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jpayne@68
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268 }
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269
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270 private static int[] makeArray(String fnames){
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271 String[] split;
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272 if(new File(fnames).exists()){split=new String[] {fnames};}
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273 else if(fnames.indexOf(',')>=0){split=fnames.split(",");}
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274 else if(fnames.indexOf('#')>=0){
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275 assert(fnames.indexOf("/")<0) : "Note: Wildcard # only works for relative paths in present working directory.";
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276 File dir=new File(System.getProperty("user.dir"));
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277 String prefix=fnames.substring(0, fnames.indexOf('#'));
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278 String suffix=fnames.substring(fnames.indexOf('#')+1);
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279
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280 File[] array=dir.listFiles();
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281 StringBuilder sb=new StringBuilder();
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jpayne@68
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282 String comma="";
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jpayne@68
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283 for(File f : array){
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284 String s=f.getName();
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jpayne@68
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285 if(s.startsWith(prefix) && s.startsWith(suffix)){
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286 sb.append(comma);
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287 sb.append(s);
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288 comma=",";
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289 }
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290 }
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291 split=sb.toString().split(",");
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jpayne@68
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292 }else{
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293 throw new RuntimeException("Invalid file: "+fnames);
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jpayne@68
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294 }
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295
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296 IntList list=new IntList();
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jpayne@68
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297 // assert(max<Integer.MAX_VALUE) : "Overflow.";
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jpayne@68
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298 // int[] x=new int[(int)max+1];
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jpayne@68
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299 // Arrays.fill(x, -1);
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300
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301 long total=0;
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302 for(String s : split){
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303 long count=addToList(s, list);
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304 total+=count;
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jpayne@68
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305 }
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jpayne@68
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306 return list.shrink().array;
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jpayne@68
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307 }
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308
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jpayne@68
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309 private static long addToList(String fname, IntList list){
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jpayne@68
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310 boolean warned=false;
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jpayne@68
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311 ByteFile bf=ByteFile.makeByteFile(fname, true);
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312 long count=0, invalid=0;
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jpayne@68
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313 byte[] line=bf.nextLine();
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jpayne@68
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314 while(line!=null){
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315 if(line.length>0 && Tools.isDigit(line[line.length-1])){//Invalid lines will end with tab or na
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316 count++;
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jpayne@68
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317 int tab2=Tools.indexOfNth(line, '\t', 2);
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318 int tab3=Tools.indexOfNth(line, '\t', 1, tab2+1);
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jpayne@68
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319 assert(tab2>0 && (tab2<tab3) && tab3<line.length) : tab2+", "+tab3+", "+line.length;
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320 assert(tab2<line.length && line[tab2]=='\t') : tab2+", "+tab3+", '"+new String(line)+"'";
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jpayne@68
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321 assert(tab3<line.length && line[tab3]=='\t') : tab2+", "+tab3+", '"+new String(line)+"'";
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322 // assert(false) : tab2+", "+tab3+", '"+new String(line)+"'";
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323 int tid=Parse.parseInt(line, tab2+1, tab3);
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324 int gi=Parse.parseInt(line, tab3+1, line.length);
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325 if(gi>=Shared.MAX_ARRAY_LEN || gi<0){//A gi over 2.5b was observed May 3, 2021.
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326 invalid++;
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jpayne@68
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327 }else{
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328 assert(gi>=0) : "tid="+tid+", gi="+gi+", line=\n'"+new String(line)+"'";
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329 int old=list.get(gi);
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330 assert(old==0 || old==tid) : "Contradictory entries for gi "+gi+": "+old+" -> "+tid+"\n'"+new String(line)+"'\ntab2="+tab2+", tab3="+tab3;
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331
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332 list.set(gi, tid);
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333
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334 //assert(x[gi]==-1 || x[gi]==ncbi) : "Contradictory entries for gi "+gi+": "+x[gi]+" -> "+ncbi;
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335 // if(x[gi]!=-1 && x[gi]!=ncbi){
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336 // if(!warned){
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jpayne@68
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337 // System.err.println("***WARNING*** For file "+fname+":\n"+
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338 // ("Contradictory entries for gi "+gi+": mapped to both taxID "+x[gi]+" and taxID "+ncbi)+
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339 // "\nThis may be an error from NCBI and you may wish to report it, but it is\n"
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jpayne@68
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340 // + "being suppressed because NCBI data is known to contain multi-mapped gi numbers,\n"
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jpayne@68
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341 // + "at least between nucleotide and protein, and gi numbers are deprecated anyway.");
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jpayne@68
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342 // warned=true;
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jpayne@68
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343 // }
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jpayne@68
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344 // }else{
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345 // x[gi]=ncbi;
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346 // }
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347 }
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348 }else{
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349 if(line.length==0){System.err.println(fname+", "+count);}//debug
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350 invalid++;
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351 }
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352 line=bf.nextLine();
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353 }
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354 if(verbose){System.err.println("Count: "+count+"; \tInvalid: "+invalid);}
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355 bf.close();
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356 return count;
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357 }
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358
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359 // private static int[] makeArrayOld(String fnames){
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360 // String[] split;
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jpayne@68
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361 // if(new File(fnames).exists()){split=new String[] {fnames};}
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jpayne@68
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362 // else{split=fnames.split(",");}
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jpayne@68
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363 //
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364 // long max=0;
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jpayne@68
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365 // for(String s : split){
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jpayne@68
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366 // max=Tools.max(max, findMaxID(s));
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jpayne@68
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367 // }
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jpayne@68
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368 //
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369 // assert(max<Integer.MAX_VALUE) : "Overflow.";
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jpayne@68
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370 // int[] x=new int[(int)max+1];
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jpayne@68
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371 // Arrays.fill(x, -1);
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jpayne@68
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372 //
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373 // long total=0;
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jpayne@68
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374 // for(String s : split){
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jpayne@68
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375 // long count=fillArray(s, x);
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jpayne@68
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376 // total+=count;
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jpayne@68
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377 // }
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jpayne@68
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378 // return x;
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jpayne@68
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379 // }
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jpayne@68
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380 //
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381 // private static long findMaxID(String fname){
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jpayne@68
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382 // ByteFile bf=ByteFile.makeByteFile(fname, true);
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jpayne@68
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383 // long count=0, max=0;
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jpayne@68
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384 // byte[] line=bf.nextLine();
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jpayne@68
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385 // while(line!=null){
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jpayne@68
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386 // count++;
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jpayne@68
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387 // int tab=Tools.indexOf(line, (byte)'\t');
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jpayne@68
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388 // long gi=Parse.parseLong(line, 0, tab);
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jpayne@68
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389 // max=Tools.max(max, gi);
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jpayne@68
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390 // line=bf.nextLine();
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jpayne@68
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391 // }
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jpayne@68
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392 // bf.close();
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jpayne@68
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393 // return max;
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jpayne@68
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394 // }
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jpayne@68
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395 //
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jpayne@68
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396 // private static long fillArray(String fname, int[] x){
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jpayne@68
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397 // boolean warned=false;
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jpayne@68
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398 // ByteFile bf=ByteFile.makeByteFile(fname, true);
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jpayne@68
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399 // long count=0;
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jpayne@68
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400 // byte[] line=bf.nextLine();
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jpayne@68
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401 // while(line!=null){
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jpayne@68
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402 // count++;
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jpayne@68
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403 // int tab=Tools.indexOf(line, (byte)'\t');
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jpayne@68
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404 // int gi=Parse.parseInt(line, 0, tab);
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jpayne@68
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405 // int ncbi=Parse.parseInt(line, tab+1, line.length);
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jpayne@68
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406 // //assert(x[gi]==-1 || x[gi]==ncbi) : "Contradictory entries for gi "+gi+": "+x[gi]+" -> "+ncbi;
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jpayne@68
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407 // if(x[gi]!=-1 && x[gi]!=ncbi){
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jpayne@68
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408 // if(!warned){
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jpayne@68
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409 // System.err.println("***WARNING*** For file "+fname+":\n"+
|
jpayne@68
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410 // ("Contradictory entries for gi "+gi+": mapped to both taxID "+x[gi]+" and taxID "+ncbi)+
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jpayne@68
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411 // "\nThis may be an error from NCBI and you may wish to report it, but it is\n"
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jpayne@68
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412 // + "being suppressed because NCBI data is known to contain multi-mapped gi numbers,\n"
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jpayne@68
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413 // + "at least between nucleotide and protein, and gi numbers are deprecated anyway.");
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jpayne@68
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414 // warned=true;
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jpayne@68
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415 // }
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jpayne@68
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416 // }else{
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jpayne@68
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417 // x[gi]=ncbi;
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jpayne@68
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418 // }
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jpayne@68
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419 // line=bf.nextLine();
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jpayne@68
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420 // }
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jpayne@68
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421 // if(verbose){System.err.println("Count: "+count);}
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jpayne@68
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422 // bf.close();
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jpayne@68
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423 // return count;
|
jpayne@68
|
424 // }
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jpayne@68
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425
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jpayne@68
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426 private static int[] array;
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jpayne@68
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427 private static String fileString;
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jpayne@68
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428
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jpayne@68
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429 public static boolean verbose=false;
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jpayne@68
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430 private static boolean initialized=false;
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jpayne@68
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431 }
|