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author jpayne
date Tue, 18 Mar 2025 16:23:26 -0400
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jpayne@68 1 .TH wgsim 1 "21 June 2017" "samtools-1.5" "Bioinformatics tools"
jpayne@68 2 .SH NAME
jpayne@68 3 wgsim \- Whole-genome sequencing read simulator
jpayne@68 4 .SH SYNOPSIS
jpayne@68 5 .B wgsim
jpayne@68 6 [\fI\,options\/\fR] \fI\,<in.ref.fa> <out.read1.fq> <out.read2.fq>\/\fR
jpayne@68 7 .PP
jpayne@68 8 <in.ref.fa> must be a fasta file containing a reference genome.
jpayne@68 9 .PP
jpayne@68 10 <out.read1.fq> and <out.read2.fq> are the first and second read output files.
jpayne@68 11 .SH OPTIONS
jpayne@68 12 .TP
jpayne@68 13 \fB\-e\fR FLOAT
jpayne@68 14 base error rate [0.000]
jpayne@68 15 .TP
jpayne@68 16 \fB\-d\fR INT
jpayne@68 17 outer distance between the two ends [500]
jpayne@68 18 .TP
jpayne@68 19 \fB\-s\fR INT
jpayne@68 20 standard deviation [50]
jpayne@68 21 .TP
jpayne@68 22 \fB\-N\fR INT
jpayne@68 23 number of read pairs [1000000]
jpayne@68 24 .TP
jpayne@68 25 \fB\-1\fR INT
jpayne@68 26 length of the first read [70]
jpayne@68 27 .TP
jpayne@68 28 \fB\-2\fR INT
jpayne@68 29 length of the second read [70]
jpayne@68 30 .TP
jpayne@68 31 \fB\-r\fR FLOAT
jpayne@68 32 rate of mutations [0.0010]
jpayne@68 33 .TP
jpayne@68 34 \fB\-R\fR FLOAT
jpayne@68 35 fraction of indels [0.15]
jpayne@68 36 .TP
jpayne@68 37 \fB\-X\fR FLOAT
jpayne@68 38 probability an indel is extended [0.30]
jpayne@68 39 .TP
jpayne@68 40 \fB\-S\fR INT
jpayne@68 41 seed for random generator [\-1]
jpayne@68 42 .TP
jpayne@68 43 \fB\-A\fR FLOAT
jpayne@68 44 discard if the fraction of ambiguous bases higher than FLOAT [0.05]
jpayne@68 45 .TP
jpayne@68 46 \fB\-h\fR
jpayne@68 47 haplotype mode
jpayne@68 48 .TP
jpayne@68 49 Parameter defaults are given in square brackets.
jpayne@68 50 .SH AUTHOR
jpayne@68 51 Copyright 2008 Genome Research Limited.
jpayne@68 52 .br
jpayne@68 53 Copyright 2011 Heng Li.
jpayne@68 54 .TP
jpayne@68 55 wgsim is part of samtools, https://github.com/samtools/samtools