Mercurial > repos > rliterman > csp2
comparison CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/opt/bbmap-39.01-1/crosscontaminate.sh @ 69:33d812a61356
planemo upload commit 2e9511a184a1ca667c7be0c6321a36dc4e3d116d
author | jpayne |
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date | Tue, 18 Mar 2025 17:55:14 -0400 |
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67:0e9998148a16 | 69:33d812a61356 |
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1 #!/bin/bash | |
2 | |
3 usage(){ | |
4 echo " | |
5 Written by Brian Bushnell | |
6 Last modified February 17, 2015 | |
7 | |
8 Description: Generates synthetic cross-contaminated files from clean files. | |
9 Intended for use with synthetic reads generated by SynthMDA or RandomReads. | |
10 | |
11 Usage: crosscontaminate.sh in=<file,file,...> out=<file,file,...> | |
12 | |
13 Input parameters: | |
14 in=<file,file,...> Clean input reads. | |
15 innamefile=<file> A file containing the names of input files, | |
16 one name per line. | |
17 interleaved=auto (int) t/f overrides interleaved autodetection. | |
18 qin=auto Input quality offset: 33 (Sanger), 64, or auto. | |
19 reads=-1 If positive, quit after processing X reads or pairs. | |
20 | |
21 Processing Parameters: | |
22 minsinks=1 Min contamination destinations from one source. | |
23 maxsinks=8 Max contamination destinations from one source. | |
24 minprob=0.000005 Min allowed contamination rate (geometric distribution). | |
25 maxprob=0.025 Max allowed contamination rate. | |
26 | |
27 Output parameters: | |
28 out=<file,file,...> Contaminated output reads. | |
29 outnamefile=<file> A file containing the names of output files, | |
30 one name per line. | |
31 overwrite=t (ow) Grant permission to overwrite files. | |
32 #showspeed=t (ss) 'f' suppresses display of processing speed. | |
33 ziplevel=2 (zl) Compression level; 1 (min) through 9 (max). | |
34 threads=auto (t) Set number of threads to use; default is number of | |
35 logical processors. | |
36 qout=auto Output quality offset: 33 (Sanger), 64, or auto. | |
37 shuffle=f Shuffle contents of output files. | |
38 shufflethreads=3 Use this many threads for shuffling (uses more memory). | |
39 | |
40 Java Parameters: | |
41 -Xmx This will set Java's memory usage, overriding autodetection. | |
42 -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will specify 200 megs. | |
43 The max is typically 85% of physical memory. | |
44 -eoom This flag will cause the process to exit if an | |
45 out-of-memory exception occurs. Requires Java 8u92+. | |
46 -da Disable assertions. | |
47 | |
48 Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. | |
49 " | |
50 } | |
51 | |
52 #This block allows symlinked shellscripts to correctly set classpath. | |
53 pushd . > /dev/null | |
54 DIR="${BASH_SOURCE[0]}" | |
55 while [ -h "$DIR" ]; do | |
56 cd "$(dirname "$DIR")" | |
57 DIR="$(readlink "$(basename "$DIR")")" | |
58 done | |
59 cd "$(dirname "$DIR")" | |
60 DIR="$(pwd)/" | |
61 popd > /dev/null | |
62 | |
63 #DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" | |
64 CP="$DIR""current/" | |
65 | |
66 z="-Xmx4g" | |
67 z2="-Xms4g" | |
68 set=0 | |
69 | |
70 if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then | |
71 usage | |
72 exit | |
73 fi | |
74 | |
75 calcXmx () { | |
76 source "$DIR""/calcmem.sh" | |
77 setEnvironment | |
78 parseXmx "$@" | |
79 if [[ $set == 1 ]]; then | |
80 return | |
81 fi | |
82 freeRam 4000m 42 | |
83 z="-Xmx${RAM}m" | |
84 } | |
85 calcXmx "$@" | |
86 | |
87 crosscontaminate() { | |
88 local CMD="java $EA $EOOM $z -cp $CP jgi.CrossContaminate $@" | |
89 echo $CMD >&2 | |
90 eval $CMD | |
91 } | |
92 | |
93 crosscontaminate "$@" |