Mercurial > repos > rliterman > csp2
diff CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/opt/bbmap-39.01-1/crosscontaminate.sh @ 69:33d812a61356
planemo upload commit 2e9511a184a1ca667c7be0c6321a36dc4e3d116d
author | jpayne |
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date | Tue, 18 Mar 2025 17:55:14 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/CSP2/CSP2_env/env-d9b9114564458d9d-741b3de822f2aaca6c6caa4325c4afce/opt/bbmap-39.01-1/crosscontaminate.sh Tue Mar 18 17:55:14 2025 -0400 @@ -0,0 +1,93 @@ +#!/bin/bash + +usage(){ +echo " +Written by Brian Bushnell +Last modified February 17, 2015 + +Description: Generates synthetic cross-contaminated files from clean files. +Intended for use with synthetic reads generated by SynthMDA or RandomReads. + +Usage: crosscontaminate.sh in=<file,file,...> out=<file,file,...> + +Input parameters: +in=<file,file,...> Clean input reads. +innamefile=<file> A file containing the names of input files, + one name per line. +interleaved=auto (int) t/f overrides interleaved autodetection. +qin=auto Input quality offset: 33 (Sanger), 64, or auto. +reads=-1 If positive, quit after processing X reads or pairs. + +Processing Parameters: +minsinks=1 Min contamination destinations from one source. +maxsinks=8 Max contamination destinations from one source. +minprob=0.000005 Min allowed contamination rate (geometric distribution). +maxprob=0.025 Max allowed contamination rate. + +Output parameters: +out=<file,file,...> Contaminated output reads. +outnamefile=<file> A file containing the names of output files, + one name per line. +overwrite=t (ow) Grant permission to overwrite files. +#showspeed=t (ss) 'f' suppresses display of processing speed. +ziplevel=2 (zl) Compression level; 1 (min) through 9 (max). +threads=auto (t) Set number of threads to use; default is number of + logical processors. +qout=auto Output quality offset: 33 (Sanger), 64, or auto. +shuffle=f Shuffle contents of output files. +shufflethreads=3 Use this many threads for shuffling (uses more memory). + +Java Parameters: +-Xmx This will set Java's memory usage, overriding autodetection. + -Xmx20g will specify 20 gigs of RAM, and -Xmx200m will specify 200 megs. + The max is typically 85% of physical memory. +-eoom This flag will cause the process to exit if an + out-of-memory exception occurs. Requires Java 8u92+. +-da Disable assertions. + +Please contact Brian Bushnell at bbushnell@lbl.gov if you encounter any problems. +" +} + +#This block allows symlinked shellscripts to correctly set classpath. +pushd . > /dev/null +DIR="${BASH_SOURCE[0]}" +while [ -h "$DIR" ]; do + cd "$(dirname "$DIR")" + DIR="$(readlink "$(basename "$DIR")")" +done +cd "$(dirname "$DIR")" +DIR="$(pwd)/" +popd > /dev/null + +#DIR="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )/" +CP="$DIR""current/" + +z="-Xmx4g" +z2="-Xms4g" +set=0 + +if [ -z "$1" ] || [[ $1 == -h ]] || [[ $1 == --help ]]; then + usage + exit +fi + +calcXmx () { + source "$DIR""/calcmem.sh" + setEnvironment + parseXmx "$@" + if [[ $set == 1 ]]; then + return + fi + freeRam 4000m 42 + z="-Xmx${RAM}m" +} +calcXmx "$@" + +crosscontaminate() { + local CMD="java $EA $EOOM $z -cp $CP jgi.CrossContaminate $@" + echo $CMD >&2 + eval $CMD +} + +crosscontaminate "$@"